Gene Expression Data
Assay Details
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Assay
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Reference:
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J:180189
Mori N, et al., Ccdc85c encoding a protein at apical junctions of radial glia is disrupted in hemorrhagic hydrocephalus (hhy) mice. Am J Pathol. 2012 Jan;180(1):314-27
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6153970
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Gene symbol:
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Nes
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Gene name:
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nestin
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Antibody:
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anti-nestin
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Assay notes:
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Specimens were fixed in Methacarn fixative (methanol:chloroform:acetic acid = 6:3:1).
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Results
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Specimen
5G:
(close  )
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Genetic Background:
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involves: BALB/cHeA * MSM
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Mutant Allele(s):
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Ccdc85chhy/Ccdc85chhy
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Age:
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embryonic day 14.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebral cortex |
Very strong |
Regionally restricted |
5M
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5G
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Expression is in germinal zone of the cortex.
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| TS22: cerebral cortex ventricular layer |
Present |
Regionally restricted |
5M
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5G
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Expression is in possible intermediate neuronal progenitors born to radial glia.
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Specimen
5H:
(close )
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Genetic Background:
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involves: BALB/cHeA * MSM
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Mutant Allele(s):
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Ccdc85chhy/Ccdc85chhy
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Age:
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embryonic day 16.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cerebral cortex |
Very strong |
Regionally restricted |
5O
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5H
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Expression is in the parenchyma and there is more cell body staining around cells across the wall.
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Specimen
5I:
(close )
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Genetic Background:
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involves: BALB/cHeA * MSM
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Mutant Allele(s):
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Ccdc85chhy/Ccdc85chhy
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Age:
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embryonic day 18.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cerebral cortex |
Present |
Single cells |
5I
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5Q
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Expression is in a few radial glial cells, and in the subcortical area is heterotopia negative staining.
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Specimen
5J:
embryonic day 14.0
(more  )
Specimen
5J:
(close )
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Genetic Background:
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involves: BALB/cHeA * MSM
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Age:
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embryonic day 14.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: telencephalon |
Present |
Regionally restricted |
5N
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5J
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Expression is in radial glial cell bodies and their extending fibers.
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Specimen
5K:
embryonic day 16.0
(more )
Specimen
5K:
(close )
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Genetic Background:
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involves: BALB/cHeA * MSM
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Age:
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embryonic day 16.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: telencephalon |
Present |
Regionally restricted |
5K
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5P
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Expression is in radial glial cell bodies and their extending fibers.
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Specimen
5L:
embryonic day 18.0
(more )
Specimen
5L:
(close )
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Genetic Background:
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involves: BALB/cHeA * MSM
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Age:
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embryonic day 18.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: telencephalon |
Present |
Regionally restricted |
5L
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5R
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Expression is in radial glial cell bodies and their extending fibers.
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