Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:104848
Lin Y, et al., Dazl deficiency leads to embryonic arrest of germ cell development in XY C57BL/6 mice. Dev Biol. 2005 Dec 15;288(2):309-16
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:6153932
|
Gene symbol:
|
Dppa3
|
Gene name:
|
developmental pluripotency-associated 3
|
Probe:
|
Dppa3/Stella probe1
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
4E:
embryonic day 14.5
(more )
Specimen
4E:
(close )
Genetic Background:
|
involves: C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Male
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4F:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
Mutant Allele(s):
|
Dazltm1Hjc/Dazltm1Hjc
|
Age:
|
embryonic day 14.5
|
Sex:
|
Male
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: testis |
Weak |
|
4F
|
Authors report a modest but consistent decrease in expression levels in mutant gonads compared with littermate controls.
|
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
Mutant Allele(s):
|
Dazltm1Hjc/Dazltm1Hjc
|
Age:
|
embryonic day 15.5
|
Sex:
|
Male
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: testis |
Trace |
|
|
Authors state a marked reduced in expression levels was evident at E15.5.
|
|