Antibody:	anti-cubulin
Detection system:	Secondary antibody coupled to Cy5

Structure	Level	Pattern	Image	Note
TS8: extraembryonic visceral endoderm	Strong		1B
TS8: ectoplacental cone	Absent		1B
TS8: epiblast	Absent		1B

Structure	Level	Pattern	Image	Note
TS12: extraembryonic visceral endoderm	Strong		1D
TS12: embryo endoderm	Present	Regionally restricted	1D	Expression is in a population of irregularly shaped cells.
TS12: neural plate	Absent		1D

Structure	Level	Pattern	Image	Note
TS11: embryo endoderm	Present	Regionally restricted	2A	Expression is in irregular dispersed small rounded cells and concentrated along the embryonic axis.

Structure	Level	Pattern	Image	Note
TS11: embryo endoderm	Present	Regionally restricted	2B	Expression is in irregular dispersed small rounded cells and concentrated along the embryonic axis.

Structure	Level	Pattern	Image	Note
TS11: embryo endoderm	Present	Regionally restricted	2C	Expression is in irregular dispersed small rounded cells and concentrated along the embryonic axis.

Structure	Level	Pattern	Image	Note
TS12: extraembryonic visceral endoderm	Strong		3A	Expression is at the apical surface of the columnar epithelium.
TS12: embryo endoderm	Present		3A	Expression is at the apical surface.

Structure	Level	Pattern	Image	Note
TS12: embryo endoderm	Very strong	Single cells	3B/C	Expression is at the apical surface of squamous epithelium.
TS12: extraembryonic endoderm	Very strong	Single cells	3B/C	Expression is at the brush border of columnar extraembryonic endoderm.

Structure	Level	Pattern	Image	Note
TS13: extraembryonic endoderm	Very strong	Uniform	5C
TS13: embryo endoderm	Present	Scattered	5C
TS13: neural tube	Absent		5C

Structure	Level	Pattern	Image	Note
TS15: extraembryonic endoderm	Strong		6B
TS15: ectoplacental cone	Absent		6B

Structure	Level	Pattern	Image	Note
TS12: blood island	Absent