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Gene Expression Data
Assay Details
Assay
Reference: J:189956 Vogt EJ, et al., Importance of the pluripotency factor LIN28 in the mammalian nucleolus during early embryonic development. Development. 2012 Dec;139(24):4514-23
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6153156
Gene symbol: Npm1
Gene name: nucleophosmin 1
Antibody: amti-NPM (B23)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: Confocal miscroscopy.
Results
Specimen 1B: postnatal adult (more )
Note: Non-surrounded nucleolus (NSN) oocyte. Double labeled: red - Npm1; green - Lin28a (assay MGI:6153117).
Structure Level Pattern Image Note
TS28: primary oocyte Present 1C , 1B Expression is at the periphery of the nucleolus.
Specimen 1F: postnatal adult (more )
Note: Surrounded nucleolus (SN) oocyte. Double labeled: red - Npm1; green - Lin28a (assay MGI:6153117).
Structure Level Pattern Image Note
TS28: primary oocyte Present 1F , 1G Expression is absent at the periphery of the nucleolus and is diffusely distributed in the nucleoplasm.
Specimen 2B: embryonic day 0.5 (more )
Note: Zygote stage. Double labeled: red - Npm1; green - Lin28a (assay MGI:6153117).
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present 2C , 2B There is homogenous nuclear expression and is weak in the cytoplasm.
Specimen 2F: embryonic day 1.0 (more )
Note: Late 2-cell stage. Double labeled: red - Npm1; green - Lin28a (assay MGI:6153117).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present 2G , 2F Expression is at the periphery of nucleolar precursor bodies.
Specimen 2J: embryonic day 2.0 (more )
Note: 4-cell stage. Double labeled: red - Npm1; green - Lin28a (assay MGI:6153117).
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Present 2J , 2K Expression is at the periphery of nucleolar precursor bodies.
Specimen 2N: embryonic day 2.5 (more )
Note: 8-cell stage. Double labeled: red - Npm1; green - Lin28a (assay MGI:6153117).
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 2N , 2O Expression is at the periphery of nucleolar precursor bodies.
Specimen 2R: embryonic day 3.0 (more )
Note: Morula stage embryo. Double labeled: red - Npm1; green - Lin28a (assay MGI:6153117).
Structure Level Pattern Image Note
TS4: compacted morula Present 2S , 2R Expression is in nucleoli.
Specimen 2V: embryonic day 3.5 (more )
Note: Blastocyst stage embryo. Double labeled: red - Npm1; green - Lin28a (assay MGI:6153117).
Structure Level Pattern Image Note
TS5: inner cell mass Present 2W , 2V Expression is in nucleoli.
TS5: trophectoderm Present 2W , 2V Expression is in nucleoli.
Specimen S1 Ab: embryonic day 1.0 (more )
Note: Mid 2-cell stage. Double labeled: red - Npm1; green - Lin28a (assay MGI:6153117).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present S1Ab , S1Ac Expression is in the nucleoplasm.
Specimen S1 Af: embryonic day 1.0 (more )
Note: Late 2-cell stage. Double labeled: red - Npm1; green - Lin28a (assay MGI:6153117).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present S1Af , S1Ag Expression is in the nucleoplasm and at the nucleolar precursor body.
Specimen S2 Ab: postnatal adult (more )
Note: Non-surrounded nucleolus (NSN) oocyte. Double labeled: red - Npm1; green - Lin28a (assay MGI:6153117).
Structure Level Pattern Image Note
TS28: primary oocyte Present S2Ac , S2Ab Expression is at the periphery of the nucleolus.
Specimen S2 Af: postnatal adult (more )
Note: Surrounded nucleolus (SN) oocyte. Double labeled: red - Npm1; green - Lin28a (assay MGI:6153117).
Structure Level Pattern Image Note
TS28: primary oocyte Present S2Ag , S2Af Expression is absent at the periphery of the nucleolus and is diffusely distributed in the nucleoplasm.
Specimen S2 Bb: embryonic day 0.5 (more )
Note: 1-cell stage. Double labeled: red - Npm1; green - Lin28a (assay MGI:6153117).
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present S2Bc , S2Bb There is homogenous nuclear expression.
Specimen S2 Bf: embryonic day 1.0 (more )
Note: Early 2-cell stage. Double labeled: red - Npm1; green - Lin28a (assay MGI:6153117).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present S2Bf , S2Bg There is homogenous nuclear expression.
Specimen S2 Bj: embryonic day 1.0 (more )
Note: Late 2-cell stage. Double labeled: red - Npm1; green - Lin28a (assay MGI:6153117).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present S2Bj , S2Bk Expression is at the periphery of nucleolar precursor bodies.
Specimen S2 Bn: embryonic day 2.0 (more )
Note: 4-cell stage. Double labeled: red - Npm1; green - Lin28a (assay MGI:6153117).
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Present S2Bo , S2Bn Expression is at the periphery of nucleolar precursor bodies.
Specimen S3 z=3: embryonic day 3.5 (more )
Note: Blastocyst stage.
Structure Level Pattern Image Note
TS5: inner cell mass Present S3_NMP z=3 Expression is in nucleoli.
TS5: trophectoderm Present S3_NMP z=3 Expression is in nucleoli.
Specimen S3 z=10: embryonic day 3.5 (more )
Note: Blastocyst stage.
Structure Level Pattern Image Note
TS5: inner cell mass Present S3_NMP z=10 Expression is in nucleoli.
TS5: trophectoderm Present S3_NMP z=10 Expression is in nucleoli.
Specimen S3 z=17: embryonic day 3.5 (more )
Note: Blastocyst stage.
Structure Level Pattern Image Note
TS5: inner cell mass Present S3_NMP z=17 Expression is in nucleoli.
TS5: trophectoderm Present S3_NMP z=17 Expression is in nucleoli.
Specimen S3 z=22: embryonic day 3.5 (more )
Note: Blastocyst stage.
Structure Level Pattern Image Note
TS5: inner cell mass Present S3_NMP z=22 Expression is in nucleoli.
TS5: trophectoderm Present S3_NMP z=22 Expression is in nucleoli.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
MGI 6.23 		The Jackson Laboratory