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Gene Expression Data
Assay Details
Assay
Reference: J:189956 Vogt EJ, et al., Importance of the pluripotency factor LIN28 in the mammalian nucleolus during early embryonic development. Development. 2012 Dec;139(24):4514-23
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6153117
Gene symbol: Lin28a
Gene name: lin-28 homolog A
Antibody: LIN28 antibody
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: Confocal miscroscopy.
Results
Specimen 1A: postnatal adult (more )
Note: Non-surrounded nucleolus (NSN) oocyte. Double labeled: green - Lin28a; red - Npm1 (assay MGI:6153156).
Structure Level Pattern Image Note
TS28: primary oocyte Present 1C , 1A Expression is at the periphery of the nucleolus.
Specimen 1E: postnatal adult (more )
Note: Surrounded nucleolus (SN) oocyte. Double labeled: green - Lin28a; red - Npm1 (assay MGI:6153156).
Structure Level Pattern Image Note
TS28: primary oocyte Present 1E , 1G Expression is absent at the periphery of the nucleolus and is diffusely distributed in the nucleoplasm.
Specimen 2A: embryonic day 0.5 (more )
Note: Zygote stage. Double labeled: green - Lin28a; red - Npm1 (assay MGI:6153156).
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present 2A , 2C There is homogenous nuclear expression and is weak in the cytoplasm.
Specimen 2E: embryonic day 1.0 (more )
Note: Late 2-cell stage. Double labeled: green - Lin28a; red - Npm1 (assay MGI:6153156).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present 2E , 2G Expression is at the periphery of nucleolar precursor bodies.
Specimen 2I: embryonic day 2.0 (more )
Note: 4-cell stage. Double labeled: green - Lin28a; red - Npm1 (assay MGI:6153156).
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Present 2K , 2I Expression is at the periphery of nucleolar precursor bodies.
Specimen 2M: embryonic day 2.5 (more )
Note: 8-cell stage. Double labeled: green - Lin28a; red - Npm1 (assay MGI:6153156).
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 2M , 2O Expression is at the periphery of nucleolar precursor bodies.
Specimen 2Q: embryonic day 3.0 (more )
Note: Morula stage embryo. Double labeled: green - Lin28a; red - Npm1 (assay MGI:6153156).
Structure Level Pattern Image Note
TS4: compacted morula Present 2Q , 2S Expression is in nucleoli.
Specimen 2U: embryonic day 3.5 (more )
Note: Blastocyst stage embryo. Double labeled: green - Lin28a; red - Npm1 (assay MGI:6153156).
Structure Level Pattern Image Note
TS5: inner cell mass Present 2U , 2W Expression is in nucleoli.
TS5: trophectoderm Present 2U , 2W Expression is in nucleoli.
Specimen S1 Aa: embryonic day 1.0 (more )
Note: Mid 2-cell stage. Double labeled: green - Lin28a; red - Npm1 (assay MGI:6153156).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present S1Ac , S1Aa Expression is in the nucleoplasm and is weak in the cytoplasm.
Specimen S1 Ae: embryonic day 1.0 (more )
Note: Late 2-cell stage. Double labeled: green - Lin28a; red - Npm1 (assay MGI:6153156).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present S1Ae , S1Ag Expression is in the nucleoplasm and at the nucleolar precursor body.
Specimen S2 Aa: postnatal adult (more )
Note: Non-surrounded nucleolus (NSN) oocyte. Double labeled: green - Lin28a; red - Npm1 (assay MGI:6153156).
Structure Level Pattern Image Note
TS28: primary oocyte Present S2Ac , S2Aa Expression is at the periphery of the nucleolus.
Specimen S2 Ae: postnatal adult (more )
Note: Surrounded nucleolus (SN) oocyte. Double labeled: green - Lin28a; red - Npm1 (assay MGI:6153156).
Structure Level Pattern Image Note
TS28: primary oocyte Present S2Ag , S2Ae Expression is absent at the periphery of the nucleolus and is diffusely distributed in the nucleoplasm.
Specimen S2 Ba: embryonic day 0.5 (more )
Note: 1-cell stage. Double labeled: green - Lin28a; red - Npm1 (assay MGI:6153156).
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present S2Bc , S2Ba There is homogenous nuclear expression and is weak in the cytoplasm.
Specimen S2 Be: embryonic day 1.0 (more )
Note: Early 2-cell stage. Double labeled: green - Lin28a; red - Npm1 (assay MGI:6153156).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present S2Bg , S2Be There is homogenous nuclear expression and is weak in the cytoplasm.
Specimen S2 Bi: embryonic day 1.0 (more )
Note: Late 2-cell stage. Double labeled: green - Lin28a; red - Npm1 (assay MGI:6153156).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present S2Bi , S2Bk Expression is at the periphery of nucleolar precursor bodies.
Specimen S2 Bm: embryonic day 2.0 (more )
Note: 4-cell stage. Double labeled: green - Lin28a; red - Npm1 (assay MGI:6153156).
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Present S2Bm , S2Bo Expression is at the periphery of nucleolar precursor bodies.
Specimen S3 z=3: embryonic day 3.5 (more )
Note: Blastocyst stage.
Structure Level Pattern Image Note
TS5: inner cell mass Present S3_LIN28 z=3 Expression is in nucleoli.
TS5: trophectoderm Present S3_LIN28 z=3 Expression is in nucleoli.
Specimen S3 z=10: embryonic day 3.5 (more )
Note: Blastocyst stage.
Structure Level Pattern Image Note
TS5: inner cell mass Present S3_LIN28 z=10 Expression is in nucleoli.
TS5: trophectoderm Present S3_LIN28 z=10 Expression is in nucleoli.
Specimen S3 z=17: embryonic day 3.5 (more )
Note: Blastocyst stage.
Structure Level Pattern Image Note
TS5: inner cell mass Present S3_LIN28 z=17 Expression is in nucleoli.
TS5: trophectoderm Present S3_LIN28 z=17 Expression is in nucleoli.
Specimen S3 z=22: embryonic day 3.5 (more )
Note: Blastocyst stage.
Structure Level Pattern Image Note
TS5: inner cell mass Present S3_LIN28 Z=22 Expression is in nucleoli.
TS5: trophectoderm Present S3_LIN28 Z=22 Expression is in nucleoli.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory