Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Vipr2em1.1(cre)Hze
Name:	vasoactive intestinal peptide receptor 2; endonuclease-mediated mutation 1.1, Hongkui Zeng
MGI ID:	MGI:6150903
Synonyms:	Vipr2-IRES2-Cre-D, Vipr2-IRES2-Cre-Delta, Vipr2-IRES2-Cre-Deltaneo
Gene:	Vipr2  Location: Chr12:116041346-116109881 bp, + strand  Genetic Position: Chr12, 62.59 cM, cytoband F2
Alliance:	Vipr2em1.1(cre)Hze page

Germline Transmission:	Earliest citation of germline transmission: J:260362
Parent Cell Line:	G4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NCrl)F1

Allele Type:    Endonuclease-mediated (Recombinase) Mutation:    Insertion   Vipr2em1.1(cre)Hze expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Vipr2 (MGI:107166)   Mutation details: Embryonic stem (ES) cells were used for CRISPR/Cas9 genome engineering. The donor vector sequences contained, from 5' to 3', a partial Vipr2 sequence spanning intron 12 up to and including the endogenous stop codon in exon 13, an internal ribosome entry site 2 sequence (IRES2; allows translation initiation in the middle of an mRNA sequence), a Cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK/gb2 promoter-Neomycin resistance gene-PGK polyA cassette, a frt5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 polyA signal, and an AttP site. In addition, several basepair mutations were introduced into the first 30 nucleotides of the Vipr2 3' UTR within exon 13 to protect the donor vector from CRISPR/Cas9 cleavage. Correctly targeted ES cells (Vipr2-IRES2-Cre-neo genotype) were injected into recipient blastocysts. Chimeric mice were bred to PhiC31-expressing mice to remove the AttB/AttP-flanked sequences (PGK/gb2-Neo-pA::frt5::RNA splice acceptor::3'hygro-pA) and replace it with the recombined AttB/AttP site (AttL). (J:260362)

Activity:	Tissue activity of this recombinase allele
Driver:	Vipr2 (mouse) Summary of all recombinase alleles driven by Vipr2. Comparative matrix view of recombinase activities Alliance mouse Vipr2 gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Vipr2 Mutation:	30 strains or lines available

Original:	J:260362 Daigle TL, et al., A suite of transgenic driver and reporter mouse lines with enhanced brain cell type targeting and functionality. Cell. 2018 Jul 12;174(2):465-480.e22
All:	13 reference(s)