Gli3 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:121609 Wang C, et al., The Shh-independent activator function of the full-length Gli3 protein and its role in vertebrate limb digit patterning. Dev Biol. 2007 May 15;305(2):460-9
Assay type:	Western blot
MGI Accession ID:	MGI:6149480
Gene symbol:	Gli3
Gene name:	GLI-Kruppel family member GLI3

Antibody:

anti-Gli3

Results

	Sample Information		Bands			Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Size Not Specified (b)	Size Not Specified (d)	Genetic Background	Mutant Allele(s)	Sex	Note
+/+ A	E10.5	TS17: limb bud	Present	Strong (c)	Absent	involves: 129 * C57BL/6		Not Specified	Anterior half of limb bud.
+/+ P	E10.5	TS17: limb bud	Present	Weak (c)	Absent	involves: 129 * C57BL/6		Not Specified	Posterior half of limb bud.
P1-4/P1-4 A	E10.5	TS17: limb bud	Present	Absent	Absent	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N	Gli3^tm2Blnw/Gli3^tm2Blnw	Not Specified	Anterior half of limb bud.
P1-4/P1-4 P	E10.5	TS17: limb bud	Present	Absent	Absent	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N	Gli3^tm2Blnw/Gli3^tm2Blnw	Not Specified	Posterior half of limb bud.
+/P1-4 A	E10.5	TS17: limb bud	Present	Present	Absent	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N	Gli3^tm2Blnw/Gli3⁺	Not Specified	Anterior half of limb bud.
+/P1-4 P	E10.5	TS17: limb bud	Present	Present	Absent	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N	Gli3^tm2Blnw/Gli3⁺	Not Specified	Posterior half of limb bud.
P1-4/delta699 A	E10.5	TS17: limb bud	Present	Present (e)	Present (e)	involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N	Gli3^tm1Urt/Gli3^tm2Blnw	Not Specified	Anterior half of limb bud.
P1-4/delta699 P	E10.5	TS17: limb bud	Present	Present (e)	Present (e)	involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N	Gli3^tm1Urt/Gli3^tm2Blnw	Not Specified	Posterior half of limb bud.
+/delta699 A	E10.5	TS17: limb bud	Present	Present	Present	involves: 129 * 129P2/OlaHsd * C57BL/6	Gli3^tm1Urt/Gli3⁺	Not Specified	Anterior half of limb bud.
+/delta699 P	E10.5	TS17: limb bud	Present	Present	Present	involves: 129 * 129P2/OlaHsd * C57BL/6	Gli3^tm1Urt/Gli3⁺	Not Specified	Posterior half of limb bud.
+/+ A	E10.5	TS17: limb bud	Present	Strong (c)	Absent	involves: 129 * C57BL/6		Not Specified	Anterior half of limb bud.
+/+ P	E10.5	TS17: limb bud	Present	Weak (c)	Absent	involves: 129 * C57BL/6		Not Specified	Posterior half of limb bud.

Notes:
(a) Gli3-190
(b) Gli3-83
(c) Gli3-83 repressor levels are graded from highest in the anterior to lowest in the posterior.
(d) Gli3-delta99
(e) Authors state both activator and repressor forms were expressed at similar levels among anterior and posterior limb fragments.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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