Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:101265
Kuijper S, et al., Function and regulation of Alx4 in limb development: complex genetic interactions with Gli3 and Shh. Dev Biol. 2005 Sep 15;285(2):533-44
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:6149029
|
|
Gene symbol:
|
Alx4
|
|
Gene name:
|
aristaless-like homeobox 4
|
|
Probe:
|
Alx4 cDNA7
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
|
Results
|
Specimen
3A:
embryonic day 10.5
(more  )
Specimen
3A:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
Specimen
3E:
embryonic day 10.5
(more )
Specimen
3E:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
Specimen
3K:
embryonic day 11.5
(more )
Specimen
3K:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
Specimen
3M:
embryonic day 10.5
(more )
Specimen
3M:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
Specimen
5A:
embryonic day 10.5
(more )
Specimen
5A:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
5A
|
Proximal part of expression domain extending into flank.
|
Specimen
5B:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Shhtm1Chg/Shhtm1Chg
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Widespread |
5B
|
Expression is upregulated and expanded throughout limb bud
|
Specimen
5C:
(close )
|
Genetic Background:
|
involves: C3H/HeJ * C57BL/6
|
|
Mutant Allele(s):
|
Gli3Xt-J/Gli3Xt-J
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
5C
|
Authors state proximal anterior expression is present, but medial anterior expression is downregulated.
|
Specimen
5D:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C3H/FeJLe * C57BL/6J
|
|
Mutant Allele(s):
|
Gli3Xt-J/Gli3Xt-J
Shhtm1Chg/Shhtm1Chg
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
5D
|
Proximal anterior expression is slightly more downregulated compared to Gli3-/- single mutant.
|
|
Analysis Tools