Gene Expression Data
Assay Details
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Assay
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Reference:
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J:253968
Abdelhamed Z, et al., A mutation in Ccdc39 causes neonatal hydrocephalus with abnormal motile cilia development in mice. Development. 2018 Jan 9;145(1):dev154500
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6147587
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Gene symbol:
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Dnali1
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Gene name:
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dynein, axonemal, light intermediate polypeptide 1
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Antibody:
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Dnali1 antibody
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
S4 E16.5 DNALI1:
embryonic day 16.5
(more  )
Note: Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
Specimen
S4 E16.5 DNALI1:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
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Specimen
S4 P0 DNALI1:
postnatal day 0
(more )
Note: Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
Specimen
S4 P0 DNALI1:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
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Specimen
6A WT DNALI1:
postnatal day 1
(more )
Note: Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
Specimen
6A WT DNALI1:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
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Specimen
6A prh/prh DNALI1:
postnatal day 1; Ccdc39prh/ Ccdc39prh
(more )
Note: Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
Specimen
6A prh/prh DNALI1:
(close )
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Genetic Background:
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involves: A/J * FVB/N
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Mutant Allele(s):
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Ccdc39prh/Ccdc39prh
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
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Specimen
S5 WT DNALI1:
postnatal day 9
(more )
Note: Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
Specimen
S5 WT DNALI1:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 9
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
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Specimen
S5 prh/prh DNALI1:
postnatal day 9; Ccdc39prh/ Ccdc39prh
(more )
Note: Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
Specimen
S5 prh/prh DNALI1:
(close )
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Genetic Background:
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involves: A/J * FVB/N
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Mutant Allele(s):
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Ccdc39prh/Ccdc39prh
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Age:
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postnatal day 9
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: ependyma |
Present |
Regionally restricted |
S5_prh/prh Merged
,
S5_prh/prh DNALI1
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Expression in ependymal cells in forebrain. No expression in axoneme of mutant ependymal cilia. Abnormal cytoplasmic accumulation of expression in mutant cells.
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Specimen
S6A WT DNALI1:
postnatal day 1
(more )
Note: Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
Specimen
S6A WT DNALI1:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
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Specimen
S6A prh/prh DNALI1:
postnatal day 1; Ccdc39prh/ Ccdc39prh
(more )
Note: Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
Specimen
S6A prh/prh DNALI1:
(close )
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Genetic Background:
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involves: A/J * FVB/N
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Mutant Allele(s):
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Ccdc39prh/Ccdc39prh
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
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Specimen
S6B WT DNALI1:
postnatal day 1
(more )
Note: Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
Specimen
S6B WT DNALI1:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: choroid plexus |
Present |
Regionally restricted |
S6B WT
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Expression localized at base of cilia in both wild-type and mutant cilia.
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Specimen
S6B prh/prh DNALI1:
postnatal day 1; Ccdc39prh/ Ccdc39prh
(more )
Note: Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
Specimen
S6B prh/prh DNALI1:
(close )
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Genetic Background:
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involves: A/J * FVB/N
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Mutant Allele(s):
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Ccdc39prh/Ccdc39prh
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: choroid plexus |
Present |
Regionally restricted |
S6B prh/prh
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Expression localized at base of cilia in both wild-type and mutant cilia.
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Specimen
S7 WT DNALI1:
postnatal day 3
(more )
Note: Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
Specimen
S7 WT DNALI1:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
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Specimen
S7 prh/prh DNALI1:
postnatal day 3; Ccdc39prh/ Ccdc39prh
(more )
Note: Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
Specimen
S7 prh/prh DNALI1:
(close )
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Genetic Background:
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involves: A/J * FVB/N
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Mutant Allele(s):
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Ccdc39prh/Ccdc39prh
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Age:
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postnatal day 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - Dnali1; red - acetylated tubulin; blue - DAPI.
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Analysis Tools