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Gene Expression Data
Assay Details
Assay
Reference: J:19895 Ang SL, et al., HNF-3 beta is essential for node and notochord formation in mouse development. Cell. 1994 Aug 26;78(4):561-74
Assay type: RNA in situ
MGI Accession ID: MGI:6121415
Gene symbol: Otx2
Gene name: orthodenticle homeobox 2
Probe: Otx2 cDNA9
Probe preparation: Antisense RNA
Results
Specimen 5A: embryonic day 7.5 (more )
Note: Headfold stage. Specimen was either +/+ or +/-.
Structure Level Pattern Image Note
TS11: embryo Present Regionally restricted 5A Expression was detected in the anterior third of the embryo.
TS11: neural plate Present Regionally restricted 5A Expression was detected in the anterior third of the neural plate at the headfold stage.
Specimen 5B: embryonic day 7.5; Foxa2tm1Jrt/Foxa2tm1Jrt (more )
Note: Headfold stage.
Structure Level Pattern Image Note
TS11: embryo Weak Regionally restricted 5B Weak expression was detected in the anterior region of some mutant embryos. Some mutants showed no expression.
Specimen 5E: embryonic day 8.5 (more )
Note: Specimen was either +/+ or +/-.
Structure Level Pattern Image Note
TS13: future midbrain Strong 5E
TS13: future prosencephalon Strong 5E
Specimen 5F: embryonic day 8.5; Foxa2tm1Jrt/Foxa2tm1Jrt (more )
Structure Level Pattern Image Note
TS13: future midbrain Absent 5F In six mutants analyzed, three showed no staining.
TS13: future prosencephalon Absent 5F In six mutants analyzed, three showed no staining.
Specimen 5G: embryonic day 8.5 (more )
Note: Double labeled: purple - Otx2; brown - En1 (assay MGI:6121420) and En2 (assay MGI:6121427). Specimen was either +/+ or +/-.
Structure Level Pattern Image Note
TS13: future midbrain Strong 5G
TS13: future prosencephalon Strong 5G
Specimen 5H: embryonic day 8.5; Foxa2tm1Jrt/Foxa2tm1Jrt (more )
Note: Double labeled: purple - Otx2; brown - En1 (assay MGI:6121420) and En2 (assay MGI:6121427).
Structure Level Pattern Image Note
TS13: embryo Weak Regionally restricted 5H In six mutants analyzed, three showed very weak staining limited to the anterior end.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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