Gene Expression Data
Assay Details
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Assay
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Reference:
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J:177132
Rapicavoli NA, et al., The long noncoding RNA Six3OS acts in trans to regulate retinal development by modulating Six3 activity. Neural Dev. 2011;6:32
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6121126
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Gene symbol:
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Six3
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Gene name:
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sine oculis-related homeobox 3
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Probe:
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Six3 probe20
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Horseradish peroxidase
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Assay notes:
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A Tyramide Signal Amplification step was used combined with an anti-digoxigenin-HRP antibody.
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Results
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Specimen
2A:
embryonic day 16.5
(more  )
Specimen
2A:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: retina |
Present |
Regionally restricted |
2A
,
2B
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Expression is in retinal progenitor cells.
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| TS24: retina inner nuclear layer |
Present |
Regionally restricted |
2B
,
2A
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Expression is in newly post-mitotic cells and is concentrated at the interface of the inner and outer neuroblastic layers.
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| TS24: retina outer nuclear layer |
Present |
Regionally restricted |
2A
,
2B
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Expression is concentrated at the interface of the inner and outer neuroblastic layers.
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Specimen
2E:
postnatal day 0.5
(more )
Specimen
2E:
(close )
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Genetic Background:
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CD-1
|
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Age:
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postnatal day 0.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Analysis Tools
