Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Prokr2em1(icre)Cfe
Name:	prokineticin receptor 2; endonuclease-mediated mutation 1, Carol F Elias
MGI ID:	MGI:6119460
Synonyms:	Prokr2-Cre
Gene:	Prokr2  Location: Chr2:132211625-132227413 bp, - strand  Genetic Position: Chr2, 64.15 cM
Alliance:	Prokr2em1(icre)Cfe page

Strain of Origin:

(C57BL/6 x SJL/J)F2

Allele Type:    Endonuclease-mediated (Recombinase) Mutation:    Insertion   Prokr2em1(icre)Cfe expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Prokr2 (MGI:2181363)   Mutation details: Using CRISPR/Cas9 technology, a GSG linker, a T2A viral peptide, an iCre recombinase, and a bovine growth hormone polyadenylation signal (bPA) were inserted between the final Prokr2 codon and termination codon. The sgRNA target was cloned into the plasmid pX330. The circular pX330 plasmidand circular donor plasmid were mixed and injected into the pronuclei of B6SJLF1 fertilized zygotes. Embryos were transferred to pseudopregnant B6D2F1 females to establish the mouse line. (J:256959)

Activity:	Tissue activity of this recombinase allele
Driver:	Prokr2 (mouse) Summary of all recombinase alleles driven by Prokr2. Alliance mouse Prokr2 gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Prokr2 Mutation:	42 strains or lines available

Original:	J:256959 Mohsen Z, et al., Sexually dimorphic distribution of Prokr2 neurons revealed by the Prokr2-Cre mouse model. Brain Struct Funct. 2017 Dec;222(9):4111-4129
All:	2 reference(s)