Probe:	Snai1 probe4
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS21: palatal shelf	Strong	Regionally restricted	1A	High levels of expression in medial part of each palatal shelf along the whole of its length.
TS21: nasal septum	Weak		1A	Weaker expression in primary palate and secondary nasal septum.
TS21: primary palate	Weak		1A	Weaker expression in primary palate and secondary nasal septum.

Structure	Level	Pattern	Image	Note
TS22: palatal shelf	Present	Regionally restricted	1B	Expression in medial part of palatal shelf. No expression at most posterior part of palatal shelves.v
TS22: primary palate	Present		1B
TS22: nasal septum	Present	Regionally restricted	1B	Expression in secondary nasal septum.

Structure	Level	Pattern	Image	Note
TS23: palatal shelf	Present	Regionally restricted	1C	Expression confined to medial part of anterior half of palatal shelf. No expression at most posterior part of palatal shelves or at midline.
TS23: primary palate	Present		1C

Structure	Level	Pattern	Image	Note
TS23: palatal shelf	Present	Regionally restricted	1D	No expression at most posterior part of palatal shelves or at midline.

Structure	Level	Pattern	Image	Note
TS21: palatal shelf mesenchyme	Present	Regionally restricted	1E	Expression in core of loose mesenchymal cells.
TS21: palatal shelf epithelium	Present	Regionally restricted	1E	No expression in medial edge epithelium.

Structure	Level	Pattern	Image	Note
TS22: palatal shelf mesenchyme	Present	Regionally restricted	1G	Expression in core of loose mesenchymal cells.
TS22: palatal shelf epithelium	Present	Regionally restricted	1G	No expression in medial edge epithelium.

Structure	Level	Pattern	Image	Note
TS23: palatal shelf mesenchyme	Present	Regionally restricted	1I	When palatal shelves adhere and fuse, expression concentrated in two areas at both sides of midline corresponding to condensed areas of mesenchyme.

Structure	Level	Pattern	Image	Note
TS25: secondary palate	Absent		1K	No expression in either primary or secondary palate as mesenchymal condensations begin ossification process.

Structure	Level	Pattern	Image	Note
TS23: palatal shelf epithelium	Absent		2B	No expression in palatal epithelia prior to palatal shelf adhesion.
TS23: palatal shelf mesenchyme	Strong		2B	High levels of expression in palatal mesenchyme.

Structure	Level	Pattern	Image	Note
TS23: palatal shelf epithelium	Present	Regionally restricted	2F	When midline epithelial seam (MES) has formed, expression in a few cells of medial edge epithelium (MEE) extending to epithelial triangles, but not in all MEE cells.

Structure	Level	Pattern	Image	Note
TS23: palatal shelf epithelium	Present	Regionally restricted	3B	Expression in medial edge epithelium (MEE) cells.
TS23: nasal epithelium	Present		3B	Expression in oral and nasal palatal epithelia and mesenchyme.
TS23: oral epithelium	Present		3B	Expression in oral and nasal palatal epithelia and mesenchyme.