Gene Expression Data
Assay Details
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Assay
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Reference:
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J:87407
Dudas M, et al., Tgf-beta3-induced palatal fusion is mediated by Alk-5/Smad pathway. Dev Biol. 2004 Feb 1;266(1):96-108
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6117809
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Gene symbol:
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Smad9
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Gene name:
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SMAD family member 9
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Antibody:
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anti-Phospho-Smad1/5/8
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Detection system:
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Cy3
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Assay notes:
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The antibody used recognizes the phosphorylated form of the protein. Antibody recognizes multiple gene products; Smad1 (MGI:109452) and Smad5 (MGI:1328787) have also been annotated.
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Results
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Specimen
2g:
embryonic day 14.5
(more  )
Specimen
2g:
(close  )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: palatal shelf epithelium |
Present |
Ubiquitous |
2g
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Expression in entire palatal epithelium and in the mesenchyme with no difference between wild-type and mutant embryos.
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| TS22: palatal shelf mesenchyme |
Present |
Ubiquitous |
2g
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Expression in entire palatal epithelium and in the mesenchyme with no difference between wild-type and mutant embryos.
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Specimen
2h:
embryonic day 14.5
(more )
Specimen
2h:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: palatal shelf epithelium |
Present |
Ubiquitous |
2h
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Expression in entire palatal epithelium and in the mesenchyme with no difference between wild-type and mutant embryos.
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| TS22: palatal shelf mesenchyme |
Present |
Ubiquitous |
2h
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Expression in entire palatal epithelium and in the mesenchyme with no difference between wild-type and mutant embryos.
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Specimen
2i:
embryonic day 14.5
(more )
Specimen
2i:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: palatal shelf epithelium |
Present |
Ubiquitous |
2i
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Expression in entire palatal epithelium and in the mesenchyme with no difference between wild-type and mutant embryos.
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| TS22: palatal shelf mesenchyme |
Present |
Ubiquitous |
2i
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Expression in entire palatal epithelium and in the mesenchyme with no difference between wild-type and mutant embryos.
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Specimen
2j:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Tgfb3tm1Jhg/Tgfb3tm1Jhg
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: palatal shelf epithelium |
Present |
Ubiquitous |
2j
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Expression in entire palatal epithelium and in the mesenchyme with no difference between wild-type and mutant embryos.
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| TS22: palatal shelf mesenchyme |
Present |
Ubiquitous |
2j
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Expression in entire palatal epithelium and in the mesenchyme with no difference between wild-type and mutant embryos.
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Specimen
2k:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Tgfb3tm1Jhg/Tgfb3tm1Jhg
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Agarose
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: palatal shelf epithelium |
Present |
Ubiquitous |
2k
|
Expression in entire palatal epithelium and in the mesenchyme with no difference between wild-type and mutant embryos.
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| TS22: palatal shelf mesenchyme |
Present |
Ubiquitous |
2k
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Expression in entire palatal epithelium and in the mesenchyme with no difference between wild-type and mutant embryos.
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Specimen
2l:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Tgfb3tm1Jhg/Tgfb3tm1Jhg
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: palatal shelf epithelium |
Present |
Ubiquitous |
2l
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Expression in entire palatal epithelium and in the mesenchyme with no difference between wild-type and mutant embryos.
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| TS22: palatal shelf mesenchyme |
Present |
Ubiquitous |
2l
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Expression in entire palatal epithelium and in the mesenchyme with no difference between wild-type and mutant embryos.
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Analysis Tools