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Gene Expression Data
Assay Details
Assay
Reference: J:87407 Dudas M, et al., Tgf-beta3-induced palatal fusion is mediated by Alk-5/Smad pathway. Dev Biol. 2004 Feb 1;266(1):96-108
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6117806
Gene symbol: Smad2
Gene name: SMAD family member 2
Antibody: phospho-Smad2 antibody
Detection system: Cy3
Assay notes: The antibody used recognizes the phosphorylated form of the protein.
Results
Specimen 2a: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: palatal shelf epithelium Strong Regionally restricted 2a Strong punctuated expression in the midline epithelium as well as in oral and nasal triangles.
TS22: palatal shelf mesenchyme Present Scattered 2a Scattered expression in mesenchyme of both wild-type and mutant.

Specimen 2b: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: palatal shelf epithelium Strong Regionally restricted 2b Strong punctuated expression in the midline epithelium as well as in oral and nasal triangles.
TS22: palatal shelf mesenchyme Strong Scattered 2b Scattered expression in mesenchyme of both wild-type and mutant.

Specimen 2c: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: palatal shelf epithelium Strong Regionally restricted 2c Strong punctuated expression in the midline epithelium as well as in oral and nasal triangles.
TS22: palatal shelf mesenchyme Strong Scattered 2c Scattered expression in mesenchyme of both wild-type and mutant.

Specimen 2d: embryonic day 14.5; Tgfb3tm1Jhg/Tgfb3tm1Jhg (more )
Structure Level Pattern Image Note
TS22: palatal shelf epithelium Absent 2d Authors report no expression in the midline epithelial seam of mutant embryos.
TS22: palatal shelf mesenchyme Present Scattered 2d Scattered expression in mesenchyme of both wild-type and mutant.

Specimen 2e: embryonic day 14.5; Tgfb3tm1Jhg/Tgfb3tm1Jhg (more )
Structure Level Pattern Image Note
TS22: palatal shelf epithelium Absent 2e Authors report no expression in the midline epithelial seam of mutant embryos.
TS22: palatal shelf mesenchyme Present Scattered 2e Scattered expression in mesenchyme of both wild-type and mutant.

Specimen 2f: embryonic day 14.5; Tgfb3tm1Jhg/Tgfb3tm1Jhg (more )
Structure Level Pattern Image Note
TS22: palatal shelf epithelium Absent 2f Authors report no expression in the midline epithelial seam of mutant embryos.
TS22: palatal shelf mesenchyme Present Scattered 2f Scattered expression in mesenchyme of both wild-type and mutant.

Specimen 2m: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: palatal shelf epithelium Present Regionally restricted 2m Authors report nuclear localization of phosphorylated Smad2.

Specimen 2n: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: palatal shelf epithelium Absent 2n Author state prefusion wild-type palatal shelves show no Smad2 activation.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/16/2024
MGI 6.23
The Jackson Laboratory