Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Tcrdtm1.1(cre/ERT2)Zhu
Name:	T cell receptor delta chain; targeted mutation 1.1, Yuan Zhuang
MGI ID:	MGI:6117766
Synonyms:	TCRdCreER
Gene:	Tcrd  Location: Chr14:54183530-54396655 bp, + strand  Genetic Position: Chr14, Syntenic

Germline Transmission:	Earliest citation of germline transmission: J:257202
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	129S/SvEv

Allele Type:    Targeted (Inducible, Recombinase) Inducer:    tamoxifen Mutation:    Insertion   Tcrdtm1.1(cre/ERT2)Zhu expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Tcrd (MGI:98611)   Mutation details: A targeting construct was designed to insert an internal ribosome entry site (IRES) and a creERT2 fusion gene (Cre recombinase fused to a human estrogen receptor ligand-binding domain) followed by a frt-flanked neomycin resistance (neo) cassette, just downstream of the coding region of the T cell receptor delta chain (Tcrd) gene. This construct was electroporated into 129/sv-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6J mice. Offspring were bred to C57BL/6-Tg(CAG-Flpe)2Arte mice to remove the neo cassette. Progeny was crossed to remove the Flp-expressing transgene and the resulting mice were bred to C57BL/6J inbred mice for at least 10 generations. (J:257202)

Activity:	Tissue activity of this recombinase allele
Driver:	Tcrd (mouse) Summary of all recombinase alleles driven by Tcrd. Alliance mouse Tcrd gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Tcrd Mutation:	15 strains or lines available

Original:	J:257202 Zhang B, et al., Differential Requirements of TCR Signaling in Homeostatic Maintenance and Function of Dendritic Epidermal T Cells. J Immunol. 2015 Nov 1;195(9):4282-91
All:	14 reference(s)