Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Cd180tm1c(KOMP)Wtsi
Name:	CD180 antigen; targeted mutation 1c, Wellcome Trust Sanger Institute
MGI ID:	MGI:6117108
Gene:	Cd180  Location: Chr13:102830055-102843139 bp, + strand  Genetic Position: Chr13, 54.92 cM
Alliance:	Cd180tm1c(KOMP)Wtsi page
IMPC:	Cd180 gene page

Mutant Cell Line:	EPD0195_5_D11
Germline Transmission:	Earliest citation of germline transmission: J:101977
Parent Cell Line:	JM8.N4 (ES Cell)
Strain of Origin:	C57BL/6N
Project Collection:	KOMP-CSD

Allele Type:    Targeted (Conditional ready) Mutation:    Insertion     Vector: L1L2_Bact_P   Mutation details: The L1L2_Bact_P cassette was inserted at position 102840780 of Chromosome 13 upstream of exon 3 (Build GRCm39). The cassette contains an FRT site, lacZ coding sequence, loxP site, human beta-actin promoter controlling a neomycin resistance gene, SV40 polyA, a second FRT site and a second loxP site, was inserted upstream of exon 3. Another loxP site was inserted downstream of exon 3 at position 102843438. Flp-mediated recombination removed the FRT-flanked lacZ neo cassette, leaving exon 3 floxed. Exon 3 encodes the majority of the coding sequence for the gene. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:101977)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Cd180 Mutation:	52 strains or lines available

Original:	J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:	3 reference(s)