Gene Expression Data
Assay Details
Assay
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Reference:
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J:20949
Smith TH, et al., Somite subdomains, muscle cell origins, and the four muscle regulatory factor proteins. J Cell Biol. 1994 Oct;127(1):95-105
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6117036
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Gene symbol:
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Myod1
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Gene name:
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myogenic differentiation 1
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Antibody:
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anti-MyoD
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
3B:
embryonic day 9.5
(more )
Note: 24-26 somite stage embryo.
Specimen
3B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.5
|
Sex:
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Not Specified
|
Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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24-26 somite stage embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: somite 10 |
Present |
Regionally restricted |
3B
|
Expression is ventral. In neck somites expression is dorsal (not shown).
|
TS15: somite 11 |
Present |
Regionally restricted |
3B
|
Expression is ventral. In neck somites expression is dorsal (not shown).
|
Specimen
3C:
embryonic day 9.5
(more )
Note: 24-26 somite stage embryo.
Specimen
3C:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
24-26 somite stage embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: somite 10 |
Present |
Regionally restricted |
3C
|
Expression is ventral. In neck somites expression is dorsal (not shown).
|
TS15: somite 11 |
Present |
Regionally restricted |
3C
|
Expression is ventral. In neck somites expression is dorsal (not shown).
|
Specimen
6D:
embryonic day 10.5
(more )
Specimen
6D:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: somite 11 dermomyotome |
Strong |
Regionally restricted |
6D
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Expression is strong in the ventral part and weak in the dorsal part.
|
TS17: somite 12 dermomyotome |
Strong |
Regionally restricted |
6D
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Expression is strong in the ventral part and weak in the dorsal part.
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TS17: trunk myotome |
Strong |
Regionally restricted |
6D
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Expression is strong in the ventral part and weak in the dorsal part. Expression is in all but the most caudal 4-5 somites.
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Specimen
7:
embryonic day 10.5
(more )
Specimen
7:
(close )
Genetic Background:
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CD-1
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Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: trunk dermatome |
Present |
Regionally restricted |
7_MyoD
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Expression is ventral, and in the anterior and posterior lips of the dermatome.
|
Specimen
not shown E8.5:
embryonic day 8.5
(more )
Specimen
not shown E8.5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
not shown E9:
embryonic day 9.0
(more )
Note: Less or equal than 19 somite stage embryo.
Specimen
not shown E9:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Less or equal than 19 somite stage embryo.
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Specimen
not shown E10.5:
embryonic day 10.5
(more )
Specimen
not shown E10.5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: tail somite |
Present |
Regionally restricted |
|
Expression is ventral.
|
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