Gene Expression Data
Assay Details
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Assay
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Reference:
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J:251844
Zamboni V, et al., Disruption of ArhGAP15 results in hyperactive Rac1, affects the architecture and function of hippocampal inhibitory neurons and causes cognitive deficits. Sci Rep. 2016 Oct 7;6:34877
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6115351
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Gene symbol:
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Calb2
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Gene name:
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calbindin 2
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Antibody:
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anti-Calretinin
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
2a Wild-type:
postnatal adult
(more  )
Specimen
2a Wild-type:
(close  )
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Genetic Background:
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involves: C57BL/6 * DBA
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2a ArhGAP15-/-:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * DBA
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Mutant Allele(s):
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Arhgap15tm1Ehi/Arhgap15tm1Ehi
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: dentate gyrus |
Present |
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2a ArhGAP15-/-
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Authors report a reduced number of expressing neurons in mutant compared with wild type.
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Specimen
not shown Wild-type:
postnatal adult
(more )
Specimen
not shown Wild-type:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
not shown ArhGAP15-/-:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * DBA
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Mutant Allele(s):
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Arhgap15tm1Ehi/Arhgap15tm1Ehi
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: hippocampus |
Present |
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No difference in the number of expressing neurons in the CA1-CA3 regions.
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Specimen
3e:
embryonic day 17.5
(more )
Specimen
3e:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3e':
embryonic day 17.5
(more )
Specimen
3e':
(close )
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Genetic Background:
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involves: C57BL/6 * DBA
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: hippocampus |
Present |
Regionally restricted |
3e'
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Expression in tangentially migrating immature neurons.
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Specimen
3e":
embryonic day 17.5
(more )
Specimen
3e":
(close )
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Genetic Background:
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involves: C57BL/6 * DBA
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: hippocampus |
Present |
Regionally restricted |
3e"
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Expression in tangentially migrating immature neurons.
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Specimen
3f:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * DBA
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Mutant Allele(s):
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Arhgap15tm1Ehi/Arhgap15tm1Ehi
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: hippocampus |
Present |
Regionally restricted |
3f
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Authors report there was no difference in the total number of expression cells or in their distribution.
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Specimen
3f':
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * DBA
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Mutant Allele(s):
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Arhgap15tm1Ehi/Arhgap15tm1Ehi
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: hippocampus |
Present |
Regionally restricted |
3f'
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Authors state that expressing cells in mutant deviate more than in wild-type.
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Specimen
3f":
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * DBA
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Mutant Allele(s):
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Arhgap15tm1Ehi/Arhgap15tm1Ehi
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: hippocampus |
Present |
Regionally restricted |
3f"
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Authors state that expressing cells in mutant deviate more than in wild-type.
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Analysis Tools