Ngly1tm2d(KOMP)Wtsi
Targeted Allele Detail
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Symbol: |
Ngly1tm2d(KOMP)Wtsi |
Name: |
N-glycanase 1; targeted mutation 2d, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:6114996 |
Gene: |
Ngly1 Location: Chr14:6157837-6220449 bp, - strand Genetic Position: Chr14, 7.08 cM, cytoband A2
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Alliance: |
Ngly1tm2d(KOMP)Wtsi page
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IMPC: |
Ngly1 gene page |
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Mutant Cell Line: |
EPD0044_5_F07 |
Germline Transmission: |
Earliest citation of germline transmission:
J:101977
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Parent Cell Line: |
JM8.F6 (ES Cell)
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Strain of Origin: |
C57BL/6N
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Project Collection: |
KOMP-CSD
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Allele Type: |
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Targeted (Null/knockout) |
Mutation: |
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Intragenic deletion
Vector: L1L2_gt2
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Mutation details: The L1L2_gt2 cassette was placed upstream of the critical exon (exon 2) of the gene; inserting at bp position Chr14:16,254,154 [Build GRCm38]. The cassette is composed of composed of an FRT site, a mouse En2 intron/splice-acceptor/exon (En2 SA), a self-cleaving 2A peptide, the beta-galactosidase gene (lacZ), a second self-cleaving 2A peptide, the neomycin-resistance gene (neo), an SV40 polyadenylation signal (SV40pA), a second FRT site and a loxP site. A second loxP site was inserted downstream of exon 2 at bp position Chr14:16,255,007 bp [Build GRCm38]; thereby flanking exon 2 with loxP sites. Cre-mediated recombination removed exon 2. Flp-mediated recombination removed the FRT-flanked neo cassette
(J:101977, J:148605)
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Original: |
J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009; |
All: |
2 reference(s) |
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