Asns RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:236441 Ruzzo EK, et al., Deficiency of asparagine synthetase causes congenital microcephaly and a progressive form of encephalopathy. Neuron. 2013 Oct 16;80(2):429-41
Assay type:	RT-PCR
MGI Accession ID:	MGI:6113836
Gene symbol:	Asns
Gene name:	asparagine synthetase

Probe:	Mm00803785_m1
Assay notes:	Quantitative RT-PCR. Relative levels were normalized to Gapdh.

Results

Image: 5A

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Amount	Genetic Background	Mutant Allele(s)	Sex
Asns^+/+	P m 3-4	TS28: cerebral hemisphere	Present	Not Specified; total RNA	C57BL/6N		Not Specified
Asns^+/-	P m 3-4	TS28: cerebral hemisphere	Present	Not Specified; total RNA	C57BL/6N-Asns^{tm1a(EUCOMM)Wtsi}	Asns^{tm1a(EUCOMM)Wtsi}/Asns⁺	Not Specified
Asns^-/-	P m 3-4	TS28: cerebral hemisphere	Present	Not Specified; total RNA	C57BL/6N-Asns^{tm1a(EUCOMM)Wtsi}	Asns^{tm1a(EUCOMM)Wtsi}/Asns^{tm1a(EUCOMM)Wtsi}	Not Specified

Notes:
(a) Both mutant genotypes were significantly different from wild type mRNA levels and significantly different from each other. The data demonstrate that the genetrap mouse is a hypomorph with ~20% of the normal level of mRNA being expressed.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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