Gene Expression Data
Assay Details
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Assay
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Reference:
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J:55332
Takeda S, et al., Left-right asymmetry and kinesin superfamily protein KIF3A: new insights in determination of laterality and mesoderm induction by kif3A-/- mice analysis. J Cell Biol. 1999 May 17;145(4):825-36
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6113391
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Gene symbol:
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T
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Gene name:
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brachyury, T-box transcription factor T
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Probe:
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Brachyury probe13
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
10C:
embryonic day 9.5
(more  )
Specimen
10C:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: notochord |
Strong |
Regionally restricted |
10C
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Expressed in the midline with a strong accent at the tail bud region.
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Specimen
10D:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6J
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Mutant Allele(s):
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Kif3atm1Noh/Kif3atm1Noh
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: notochord |
Present |
Regionally restricted |
10D
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Similar expression patter as wild-type. Expressed in the midline with a strong accent at the tail bud region. Expression was fainter compared with the wild-type.
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Analysis Tools
