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IghFluBI
Allele Detail
Summary
Symbol: IghFluBI
Name: immunoglobulin heavy chain complex; FluBI
MGI ID: MGI:6112033
Gene: Igh  Location: Chr12:113222388-115973574 bp, - strand  Genetic Position: Chr12, Syntenic
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:206756
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  C57BL/6
Mutation
description
Allele Type:    Not Applicable (Not Applicable)
Mutation:    Other
 
Mutation details: This germline-transmitted allele is a functionally rearranged immunoglobulin heavy chain gene from a hemagglutinin-reactive B cell of a mouse that had been immunized with influenza virus strain A/SWN/33. The variable (VDJ) portion of the expressed Igh sequence (GenBank accession KF419287.1) is derived from Igh-V1-50, Igh-DQ52, and Igh-J2 and contains 7 somatic mutations; it is joined to the Ighg2b constant region gene segment. B cells of mice bearing this allele in combination with IgkFluBI, called FluBI mice, are specific for hemagglutinin and secrete neutralizing antibodies of subtype IgG2b against A/WSN/33 influenza virus. The FluBI mouse was generated by somatic cell nuclear transfer (SCNT) of the nucleus of a B cell from an influenza strain A/SWN/3-immunized (C57BL/6 x 129)F1 mouse into an enucleated oocyte, which was allowed to develop to the blastocyst stage, then used to create embryonic stem (ES) cell lines. These ES cells were injected into blastocysts to create chimeric mice that transmitted the rearranged Igk and Igh genes, both derived from C57BL/6, in the germline. (J:206756)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Igh Mutation:  39 strains or lines available
References
Original:  J:206756 Dougan SK, et al., Antigen-specific B-cell receptor sensitizes B cells to infection by influenza virus. Nature. 2013 Nov 21;503(7476):406-9
All:  1 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory