Gene Expression Data
Assay Details
Assay
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Reference:
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J:29655
Li L, et al., Dermo-1: a novel twist-related bHLH protein expressed in the developing dermis. Dev Biol. 1995 Nov;172(1):280-92
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6112005
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Gene symbol:
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Twist2
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Gene name:
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twist basic helix-loop-helix transcription factor 2
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Probe:
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Dermo-1 probe
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
not shown E9.5:
embryonic day 9.5
(more )
Specimen
not shown E9.5:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: embryo |
Ambiguous |
|
|
Authors state expression barely detected at E9.5.
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Specimen
2A:
embryonic day 10.5
(more )
Specimen
2A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: body wall |
Present |
|
2A
|
|
TS17: forelimb bud |
Present |
|
2A
|
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TS17: embryo |
Present |
Regionally restricted |
2A
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Expression in dorsal regions of embryo overlapped with regions populated in large part by sclerotomal cells. Expression in other parts of the embryo became progressively restricted towards the embryo surface.
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Specimen
2B:
embryonic day 11.5
(more )
Specimen
2B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2C:
embryonic day 12.5
(more )
Specimen
2C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3A:
embryonic day 12.5
(more )
Specimen
3A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: embryo |
Strong |
Regionally restricted |
3A
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Very high levels of expression near the surface of the embryo and in regions containing chondrogenic cells.
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TS20: head mesenchyme |
Present |
Regionally restricted |
3A
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Expression in cranial mesenchymal cells around the nose, pharyngeal arches and tongue.
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Specimen
3B:
embryonic day 13.5
(more )
Specimen
3B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: face mesenchyme |
Strong |
Regionally restricted |
3B
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Increased expression in cranio-facial mesenchyme.
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TS21: vertebral cartilage condensation |
Present |
Regionally restricted |
3B
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In developing vertebra, expression began decreasing in rostral-to-caudal fashion, reflecting degree of differentiation of chondrogenic cells.
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TS21: vertebral pre-cartilage condensation |
Present |
Regionally restricted |
3B
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In developing vertebra, expression began decreasing in rostral-to-caudal fashion, reflecting degree of differentiation of chondrogenic cells.
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Specimen
3C:
embryonic day 15.5
(more )
Specimen
3C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: vertebra |
Present |
Regionally restricted |
3C
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Expression pattern progressively restricted to outline of caudal vertebral bodies.
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Specimen
3D:
embryonic day 16.5
(more )
Specimen
3D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: jaw |
Present |
Regionally restricted |
3D
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Expression in tongue and jaw progressively restricted to superficial regions.
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TS24: tongue |
Present |
Regionally restricted |
3D
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Expression in tongue and jaw progressively restricted to superficial regions.
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TS24: skin |
Strong |
|
3D
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In developing skin, expression intensified through 16.5 dpc.
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Specimen
4A:
embryonic day 16.5
(more )
Specimen
4A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: dermis |
Present |
Regionally restricted |
4A
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Expression localized specifically to dermal cells of epidermal-dermal junction.
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TS24: epidermis |
Absent |
|
4A
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No expression in epidermis and basal layer of skin.
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Specimen
not shown E16.5:
embryonic day 16.5
(more )
Specimen
not shown E16.5:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: hair follicle dermal papilla |
Present |
|
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When hair follicles begin to mature and invaginate into the dermis, expression in developing dermal papilla cells.
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Specimen
4B:
embryonic day 17.5
(more )
Specimen
4B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: dermis |
Present |
|
4B
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Expression in dermis increased continuously through 17.5 dpc.
|
TS25: hair follicle |
Present |
Regionally restricted |
4B
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In hair follicles, highest level of expression in dermal layer of cortex adjacent to epidermal-dermal junction. Expression only in dermal sheath. No expression in epidermis-derived central region containing the hair shaft.
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TS25: vibrissa |
Present |
|
4B
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Specimen
4C:
embryonic day 17.5
(more )
Specimen
4C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: vibrissa |
Strong |
|
4C
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Expression in whisker pad significantly higher than in surrounding skin.
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Specimen
4D:
embryonic day 17.5
(more )
Specimen
4D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: hair root sheath |
Present |
Regionally restricted |
4D
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Expression confined to dermal sheath around hair follicles. No expression in inner or outer epidermal sheaths.
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Specimen
4E:
embryonic day 15.5
(more )
Specimen
4E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: hindlimb digit |
Present |
Regionally restricted |
4E
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Expression in perichondrium of hindlimb digits.
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Specimen
4F:
embryonic day 15.5
(more )
Specimen
4F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: perichondrium |
Present |
Regionally restricted |
4F
|
Expression in perichondrium of hindlimb digits.
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Specimen
4G/H:
embryonic day 15.5
(more )
Specimen
4G/H:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: perichondrium |
Present |
Regionally restricted |
4H
,
4G
|
Expression localized in perichondrial cells surrounding cartilage of vertebrae.
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