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Gene Expression Data
Assay Details
Assay
Reference: J:29655 Li L, et al., Dermo-1: a novel twist-related bHLH protein expressed in the developing dermis. Dev Biol. 1995 Nov;172(1):280-92
Assay type: RNA in situ
MGI Accession ID: MGI:6112005
Gene symbol: Twist2
Gene name: twist basic helix-loop-helix transcription factor 2
Probe: Dermo-1 probe
Probe preparation: Antisense labelled with S35 RNA
Visualized with: Autoradiography
Results
Specimen not shown E9.5: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: embryo Ambiguous Authors state expression barely detected at E9.5.

Specimen 2A: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: body wall Present 2A
TS17: forelimb bud Present 2A
TS17: embryo Present Regionally restricted 2A Expression in dorsal regions of embryo overlapped with regions populated in large part by sclerotomal cells. Expression in other parts of the embryo became progressively restricted towards the embryo surface.

Specimen 2B: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: branchial arch Strong 2B
TS19: somite Strong Regionally restricted 2B Authors report high expression in somitic region.
TS19: dermatome Strong 2B
TS19: myotome Absent 2B Expression excluded from postmitotic muscle cells of myotome.v
TS19: sclerotome Present 2B Expression appears to mark sclerotome-derived cells, which give rise to ribs and vertebrae.

Specimen 2C: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: craniocervical region Present Regionally restricted 2C Authors report expression in branchial arches.
TS20: dermatome Present 2C
TS20: sclerotome Present 2C
TS20: myotome Absent 2C
TS20: hindlimb Present 2C

Specimen 3A: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: embryo Strong Regionally restricted 3A Very high levels of expression near the surface of the embryo and in regions containing chondrogenic cells.
TS20: head mesenchyme Present Regionally restricted 3A Expression in cranial mesenchymal cells around the nose, pharyngeal arches and tongue.

Specimen 3B: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: face mesenchyme Strong Regionally restricted 3B Increased expression in cranio-facial mesenchyme.
TS21: vertebral cartilage condensation Present Regionally restricted 3B In developing vertebra, expression began decreasing in rostral-to-caudal fashion, reflecting degree of differentiation of chondrogenic cells.
TS21: vertebral pre-cartilage condensation Present Regionally restricted 3B In developing vertebra, expression began decreasing in rostral-to-caudal fashion, reflecting degree of differentiation of chondrogenic cells.

Specimen 3C: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: vertebra Present Regionally restricted 3C Expression pattern progressively restricted to outline of caudal vertebral bodies.

Specimen 3D: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: jaw Present Regionally restricted 3D Expression in tongue and jaw progressively restricted to superficial regions.
TS24: tongue Present Regionally restricted 3D Expression in tongue and jaw progressively restricted to superficial regions.
TS24: skin Strong 3D In developing skin, expression intensified through 16.5 dpc.

Specimen 4A: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: dermis Present Regionally restricted 4A Expression localized specifically to dermal cells of epidermal-dermal junction.
TS24: epidermis Absent 4A No expression in epidermis and basal layer of skin.

Specimen not shown E16.5: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: hair follicle dermal papilla Present When hair follicles begin to mature and invaginate into the dermis, expression in developing dermal papilla cells.

Specimen 4B: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: dermis Present 4B Expression in dermis increased continuously through 17.5 dpc.
TS25: hair follicle Present Regionally restricted 4B In hair follicles, highest level of expression in dermal layer of cortex adjacent to epidermal-dermal junction. Expression only in dermal sheath. No expression in epidermis-derived central region containing the hair shaft.
TS25: vibrissa Present 4B

Specimen 4C: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: vibrissa Strong 4C Expression in whisker pad significantly higher than in surrounding skin.

Specimen 4D: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: hair root sheath Present Regionally restricted 4D Expression confined to dermal sheath around hair follicles. No expression in inner or outer epidermal sheaths.

Specimen 4E: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: hindlimb digit Present Regionally restricted 4E Expression in perichondrium of hindlimb digits.

Specimen 4F: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: perichondrium Present Regionally restricted 4F Expression in perichondrium of hindlimb digits.

Specimen 4G/H: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: perichondrium Present Regionally restricted 4H , 4G Expression localized in perichondrial cells surrounding cartilage of vertebrae.


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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory