Probe:	hoxb-9 probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: neural tube	Strong	Regionally restricted	4A	Anterior limit of expression in neural tube approximately at the level of somites 7-8 (pV3).
TS15: paraxial mesenchyme	Strong	Regionally restricted	4A	High level of expression in posterior portion of embryo; lower levels in rostral portion.
TS15: somite	Present	Regionally restricted	4A	Expression in somites detected up to the level of the 7th to 8th somite.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	4B	Anterior limit of neural tube expression shifts rostrally by one to two somites at E10.5.
TS17: spinal ganglion	Present		4B
TS17: paraxial mesenchyme	Strong	Regionally restricted	4B	Anterior limit of neural tube expression shifts rostrally by one to two somites at E10.5.
TS17: somite	Present	Regionally restricted	4B	Expression enhanced in ventral portions of somites.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Regionally restricted	4C	Authors state that changes in neural tube expression were not apparent.
TS19: paraxial mesenchyme	Weak	Regionally restricted	4C	Intensity of expression decreases progressively in anterior regions.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	4D	Authors state that changes in neural tube expression were not apparent.
TS20: rib pre-cartilage condensation	Weak		4D	Expression restricted to primordia of vertebral bodies and ribs.
TS20: vertebral pre-cartilage condensation	Weak		4D	Expression restricted to primordia of vertebral bodies and ribs.
TS20: metanephros	Present		4D	Authors report expression in kidneys from E12.5 onwards.