Antibody:

anti-neurofilament clone 2H3

Structure	Level	Pattern	Image	Note
TS20: tibial nerve	Present		3A	In wild type embryos, tibial nerve has branched and, more distally, expanded into a terminal arbor.

Structure	Level	Pattern	Image	Note
TS20: tibial nerve	Present		3B	Expression pattern resembles that of wild type.

Structure	Level	Pattern	Image	Note
TS20: tibial nerve	Present		3C	Expression pattern indicates less mature tibial nerves and more disorganized tibial arbors.

Structure	Level	Pattern	Image	Note
TS20: tibial nerve	Present		3D	Expression pattern resembles that of wild type.

Structure	Level	Pattern	Image	Note
TS20: tibial nerve	Present		3E	Expression pattern resembles that of wild type.

Structure	Level	Pattern	Image	Note
TS20: tibial nerve	Present		3F	Expression pattern indicates underdeveloped tibial arbor.

Structure	Level	Pattern	Image	Note
TS20: tibial nerve	Present		3G	Expression pattern indicates less mature tibial nerve and a more disorganized tibial arbor with fewer nerve fibers.

Structure	Level	Pattern	Image	Note
TS20: tibial nerve	Present		3H	Expression pattern indicates underdeveloped tibial arbor.

Structure	Level	Pattern	Image	Note
TS20: tibial nerve	Present		3I	Expression pattern indicates nearly complete absence of the tibial arbor.

Structure	Level	Pattern	Image	Note
TS20: deep peroneal nerve	Present		4A	Expression in superficial and deep peroneal nerves extending approximately one-third of the proximo-distal extent of the foot paddle.
TS20: sciatic nerve	Present		4A	Expression in superficial and deep peroneal nerves extending approximately one-third of the proximo-distal extent of the foot paddle.
TS20: superficial peroneal nerve	Present		4A	Expression in superficial and deep peroneal nerves extending approximately one-third of the proximo-distal extent of the foot paddle.
TS20: lateral femoral cutaneous nerve	Present		4A	Expression in lateral femoral cutaneous nerve reaching the boundary between distal limb and foot paddle.

Structure	Level	Pattern	Image	Note
TS20: deep peroneal nerve	Present		4B	Expression pattern indicates well-defined superfical and deep peroneal nerves, as well as a robust lateral femoral cutaneous nerve.
TS20: lateral femoral cutaneous nerve	Present		4B	Expression pattern indicates well-defined superfical and deep peroneal nerves, as well as a robust lateral femoral cutaneous nerve.
TS20: sciatic nerve	Present		4B	Expression pattern indicates well-defined superfical and deep peroneal nerves, as well as a robust lateral femoral cutaneous nerve.
TS20: superficial peroneal nerve	Present		4B	Expression pattern indicates well-defined superfical and deep peroneal nerves, as well as a robust lateral femoral cutaneous nerve.

Structure	Level	Pattern	Image	Note
TS20: deep peroneal nerve	Present		4C	Expression in peroneal nerves that are not as well developed as in wild type.
TS20: lateral femoral cutaneous nerve	Present		4C	Expression in peroneal nerves that are not as well developed as in wild type.
TS20: sciatic nerve	Present		4C	Expression in peroneal nerves that are not as well developed as in wild type.
TS20: superficial peroneal nerve	Present		4C	Expression in peroneal nerves that are not as well developed as in wild type.

Structure	Level	Pattern	Image	Note
TS20: deep peroneal nerve	Present		4D	Expression pattern indicates well-defined superfical and deep peroneal nerves, as well as a robust lateral femoral cutaneous nerve.
TS20: lateral femoral cutaneous nerve	Present		4D	Expression pattern indicates well-defined superfical and deep peroneal nerves, as well as a robust lateral femoral cutaneous nerve.
TS20: sciatic nerve	Present		4D	Expression pattern indicates well-defined superfical and deep peroneal nerves, as well as a robust lateral femoral cutaneous nerve.
TS20: superficial peroneal nerve	Present		4D	Expression pattern indicates well-defined superfical and deep peroneal nerves, as well as a robust lateral femoral cutaneous nerve.

Structure	Level	Pattern	Image	Note
TS20: deep peroneal nerve	Present		4E	Expression pattern indicates well-defined superfical and deep peroneal nerves, as well as a robust lateral femoral cutaneous nerve.
TS20: lateral femoral cutaneous nerve	Present		4E	Expression pattern indicates well-defined superfical and deep peroneal nerves, as well as a robust lateral femoral cutaneous nerve.
TS20: sciatic nerve	Present		4E	Expression pattern indicates well-defined superfical and deep peroneal nerves, as well as a robust lateral femoral cutaneous nerve.
TS20: superficial peroneal nerve	Present		4E	Expression pattern indicates well-defined superfical and deep peroneal nerves, as well as a robust lateral femoral cutaneous nerve.

Structure	Level	Pattern	Image	Note
TS20: deep peroneal nerve	Present		4F	Expression pattern indicates well-defined superfical and deep peroneal nerves, as well as a robust lateral femoral cutaneous nerve.
TS20: lateral femoral cutaneous nerve	Present		4F	Expression pattern indicates well-defined superfical and deep peroneal nerves, as well as a robust lateral femoral cutaneous nerve.
TS20: sciatic nerve	Present		4F	Expression pattern indicates well-defined superfical and deep peroneal nerves, as well as a robust lateral femoral cutaneous nerve.
TS20: superficial peroneal nerve	Present		4F	Expression pattern indicates well-defined superfical and deep peroneal nerves, as well as a robust lateral femoral cutaneous nerve.

Structure	Level	Pattern	Image	Note
TS20: deep peroneal nerve	Present		4G	Expression pattern indicates a thinning of the sciatic nerved and truncation of the distal peroneal nerves.
TS20: lateral femoral cutaneous nerve	Present		4G	Expression pattern indicates a thinning of the sciatic nerved and truncation of the distal peroneal nerves.
TS20: sciatic nerve	Present		4G	Expression pattern indicates a thinning of the sciatic nerved and truncation of the distal peroneal nerves.
TS20: superficial peroneal nerve	Present		4G	Expression pattern indicates a thinning of the sciatic nerved and truncation of the distal peroneal nerves.

Structure	Level	Pattern	Image	Note
TS20: deep peroneal nerve	Present		4H	Expression pattern indicates shortened peroneal nerve and moderal alterations in the lateral femoral femoral cutaneous nerve.
TS20: lateral femoral cutaneous nerve	Present		4H	Expression pattern indicates shortened peroneal nerve and moderal alterations in the lateral femoral femoral cutaneous nerve.
TS20: sciatic nerve	Present		4H	Expression pattern indicates shortened peroneal nerve and moderal alterations in the lateral femoral femoral cutaneous nerve.
TS20: superficial peroneal nerve	Present		4H	Expression pattern indicates shortened peroneal nerve and moderal alterations in the lateral femoral femoral cutaneous nerve.

Structure	Level	Pattern	Image	Note
TS20: lateral femoral cutaneous nerve	Present		4I	Expression pattern indicates a much reduced distal sciatic nerve and complete absence of superficial and deep peroneal branches.
TS20: superficial peroneal nerve	Present		4I	Expression pattern indicates a much reduced distal sciatic nerve and complete absence of superficial and deep peroneal branches.
TS20: tibial nerve	Present		4I	Expression pattern indicates a much reduced distal sciatic nerve and complete absence of superficial and deep peroneal branches.

Structure	Level	Pattern	Image	Note
TS21: tibial nerve	Present		6A	Expression in tibial nerve which has divided into two trunks prior to expansion into the distal tibial arbor, where fibers have fasciculated into 5 branches.

Structure	Level	Pattern	Image	Note
TS21: tibial nerve	Present		6B	Expression pattern resembles that seen in wild type.

Structure	Level	Pattern	Image	Note
TS21: tibial nerve	Present		6C	Expression pattern indicates a single tibial nerve trunk. Fibers in the distal tibial arbor appear defasciculated.

Structure	Level	Pattern	Image	Note
TS21: tibial nerve	Present		6D	Expression pattern indicates a single tibial nerve trunk. Terminal branches appear thinner and shorter than in wild type.

Structure	Level	Pattern	Image	Note
TS21: deep peroneal nerve	Present		7A	Expression in main trunk of sciatic nerve branching into superficial and deep peroneal nerves.
TS21: sciatic nerve	Present		7A	Expression in main trunk of sciatic nerve branching into superficial and deep peroneal nerves.
TS21: superficial peroneal nerve	Present		7A	Expression in main trunk of sciatic nerve branching into superficial and deep peroneal nerves.

Structure	Level	Pattern	Image	Note
TS21: deep peroneal nerve	Present		7B	Expression in superficial and deep peroneal nerve projections similar to thos seen in wild type.
TS21: sciatic nerve	Present		7B	Expression in superficial and deep peroneal nerve projections similar to thos seen in wild type.
TS21: superficial peroneal nerve	Present		7B	Expression in superficial and deep peroneal nerve projections similar to thos seen in wild type.

Structure	Level	Pattern	Image	Note
TS21: deep peroneal nerve	Present		7C	Expression patterns indicate much thinner sciatic nerve trunks and greatly reduced peroneal branches.
TS21: sciatic nerve	Present		7C	Expression patterns indicate much thinner sciatic nerve trunks and greatly reduced peroneal branches.
TS21: superficial peroneal nerve	Present		7C	Expression patterns indicate much thinner sciatic nerve trunks and greatly reduced peroneal branches.

Structure	Level	Pattern	Image	Note
TS21: deep peroneal nerve	Present		7D	In some cases, sciatic nerve appears not to divide distally, only a small terminal sciatic projection is evident.
TS21: sciatic nerve	Present		7D	In some cases, sciatic nerve appears not to divide distally, only a small terminal sciatic projection is evident.
TS21: superficial peroneal nerve	Present		7D	In some cases, sciatic nerve appears not to divide distally, only a small terminal sciatic projection is evident.