Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Gt(ROSA)26Sortm2(CAG-mGCaMP3)Dbe
Name:	gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Dwight E Bergles
MGI ID:	MGI:6111492
Synonyms:	Rosa26-lsl-mGCaMP3
Gene:	Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance:	Gt(ROSA)26Sortm2(CAG-mGCaMP3)Dbe page

Germline Transmission:	Earliest citation of germline transmission: J:254093
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	129

Allele Type:    Targeted (Conditional ready, Reporter) Mutation:    Insertion   Mutation details: The targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP site, an FRT-flanked neomycin resistance cassette in reverse orientation to the gene, a STOP cassette (3x SV40 polyA), a second loxP site, a GCaMP3 fusion gene, and a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability) followed by a BGH polyA signal. The GCaMP3 fusion gene contains a cDNA sequence encoding the chicken smooth muscle M13 fragment of myosin light chain kinase, a circularly permutated EGFP (amino acid residues 149-238 followed by amino acid residues 1-144 (including M153K, V163A, S175G, D180Y, T203V, A206K, and V93I mutations to increase dynamic range and baseline fluorescence), and a DNA fragment encoding amino acid residues 2-148 of the rat calmodulin (modified to harbor the N60D mutation to increase the fluorescence change for small calcium transients). To localize GCaMP3 to the plasma membrane, a gene sequence encoding the first 8 amino acids of the modified Marcks (myristoylated alanine rich protein kinase C substrate) sequence (MGCCFSKT) was fused to the transcription initiation codon. The targeting vector is inserted between exons 1 and 2. (J:254093)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:	944 strains or lines available

Original:	J:254093 Agarwal A, et al., Transient Opening of the Mitochondrial Permeability Transition Pore Induces Microdomain Calcium Transients in Astrocyte Processes. Neuron. 2017 Feb 8;93(3):587-605.e7
All:	1 reference(s)