Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Gt(ROSA)26Sorem1(CAG-cas9*,-EGFP)Dvs
Name:	gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, David V Serreze
MGI ID:	MGI:6111351
Synonyms:	Rosa26CAG-LSL-Cas9-P2A-EGFP
Gene:	Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance:	Gt(ROSA)26Sorem1(CAG-cas9*,-EGFP)Dvs page

Strain of Origin:

NOD/ShiLtJ

Allele Type:    Endonuclease-mediated (Conditional ready, Endonuclease, Reporter) Mutation:    Insertion   Mutation details: Sequences include CMV enhancer/chicken beta-actin promoter (CAG), a loxP-STOP(3xSV40 polyA)-loxP cassette (LSL), a 3xFLAG sequence, a mammalian codon-optimized cas9 gene (derived from Streptococcus pyogenes CRISPR associated protein 9 [SpCas9]), a P2A ribosomal skip cleavage peptide sequence, an enhanced green fluorescent protein gene (EGFP), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) and a bovine growth hormone polyA sequence (BGHpA). To facilitate insertion into the Gt(ROSA)26Sor locus on chromosome 6 via homology directed repair (HDR), the LSL-Cas9-P2A-EGFP sequences were co-injected with a large piece of Gt(ROSA)26Sor locus homologous DNA. (J:101977)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:	942 strains or lines available

Original:	J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017;
All:	4 reference(s)