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Gene Expression Data
Assay Details
Assay
Reference: J:116227 Carotenuto P, et al., PRUNE and NM23-M1 expression in embryonic and adult mouse brain. J Bioenerg Biomembr. 2006 Aug;38(3-4):233-46
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6110848
Gene symbol: Prune1
Gene name: prune exopolyphosphatase
Antibody: Anti-PRUNE (A59)
Detection system: Secondary antibody coupled to rhodamine
Assay notes: Two antibodies were described; the one used in this assay was not detailed. Either MGI:6110137 or MGI:6110139 could have been used.
Results
Specimen 2G: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: hippocampus Present 2G
TS22: lateral ganglionic eminence Present 2G
TS22: medial ganglionic eminence Present 2G
TS22: telencephalon ventricular layer Present 2G
Specimen not shown: postnatal adult (more )
Structure Level Pattern Image Note
TS28: basal forebrain Absent
Specimen 4A,C: postnatal adult (more )
Structure Level Pattern Image Note
TS28: cortical layer I Strong 4A , 4C
TS28: cortical layer II Strong 4A , 4C
TS28: cortical layer IV Strong 4A , 4C
TS28: neocortex layer Present Widespread 4C , 4A
Specimen 4E: postnatal adult (more )
Structure Level Pattern Image Note
TS28: dentate gyrus Present 4E
TS28: hippocampus CA1 Present 4E
TS28: hippocampus CA2 Present 4E
TS28: hippocampus CA3 Present 4E
Specimen 4G: postnatal adult (more )
Note: Double labeled: red - Prune1; blue - Dapi.
Structure Level Pattern Image Note
TS28: dentate gyrus Present 4G
TS28: hippocampus CA3 Present 4G
Specimen 4I: postnatal adult (more )
Note: Double labeled: red - Prune1; green - Tubb3 (assay MGI:6110856).
Structure Level Pattern Image Note
TS28: hippocampus CA1 Present 4I Expression was co-localized with Tubb3 predominantly in the cell bodies.
Specimen 7A,C: postnatal adult (more )
Note: Double labeled: red - Prune1; green - Nme1 (assay MGI:6110854).
Structure Level Pattern Image Note
TS28: substantia nigra Strong 7A , 7C There was overlapping staining with Nme1.
Specimen 7D,F: postnatal adult (more )
Note: Double labeled: red - Prune1; green - Th (assay MGI:6110855).
Structure Level Pattern Image Note
TS28: substantia nigra Present 7D , 7F There was overlapping staining with Th, but not all of the neurons are double labeled.
Specimen 7G,I: postnatal adult (more )
Note: Double labeled: red - Prune1; green - Nme1 (assay MGI:6110854).
Structure Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Present 7G , 7I There was overlapping staining with Nme1.
TS28: cerebellum molecular layer Present 7G , 7I There was overlapping staining with Nme1.
Specimen 7J,L: postnatal adult (more )
Note: Double labeled: red - Prune1; green - Tubb3 (assay MGI:6110856).
Structure Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Present 7J , 7L There was overlapping staining with Tubb3.
TS28: cerebellum internal granule cell layer Present 7J , 7L There was overlapping staining with Tubb3.
TS28: cerebellum molecular layer Present 7J , 7L There was overlapping staining with Tubb3.
Specimen 7M,O: postnatal adult (more )
Note: Double labeled: red - Prune1; green - Calb1 (assay MGI:6110857).
Structure Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Present 7M , 7O There was overlapping staining with Calb1.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory