Probe:	NM23-M1 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Sections were post-fixed in 4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Strong		1D	Very high expression was detected in telencephalic vesicles.
TS17: diencephalon	Present		1D

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Strong		1E	Very high expression was detected in telencephalic vesicles, the primordium of the subpallium, and the hippocampal area.
TS17: midbrain roof plate	Strong		1E
TS17: diencephalon	Present		1E
TS17: hindbrain	Strong	Regionally restricted	1E	Expression was strong in the rhombic lips of the cranial edge of the thinned roof of the fourth ventricle.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Strong		1F	Very high expression was detected in telencephalic vesicles.
TS17: midbrain roof plate	Strong		1F
TS17: hindbrain	Strong	Regionally restricted	1F	Expression was strong in the rhombic lips of the cranial edge of the thinned roof of the fourth ventricle.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Present		2B	Expression was detected in the neopallial cortex in many densely packed cells.
TS22: epithalamus	Strong		2B
TS22: hypothalamus	Strong		2B
TS22: midbrain lateral wall	Very strong		2B
TS22: cerebellum	Present		2B
TS22: midbrain tegmentum	Present		2B

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present		2D	Expression was detected in all layers of the developing telencephalon.
TS22: corpus striatum	Strong		2D
TS22: epithalamus	Strong		2D
TS22: hypothalamus	Strong		2D
TS22: midbrain lateral wall	Very strong		2D
TS22: cerebellum	Present		2D
TS22: midbrain tegmentum	Present		2D

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present		2F	Expression was detected in all layers of the developing telencephalon.
TS22: cerebral cortex intermediate zone	Present		2F
TS22: cerebral cortex marginal layer	Present		2F
TS22: cerebral cortex ventricular layer	Present		2F
TS22: cortical plate	Present		2F
TS22: cortical subplate	Present		2F
TS22: cerebral cortex	Strong		2F	Strong expression was seen in all of the cell bodies forming the cortical developing layers.
TS22: lateral ganglionic eminence	Strong		2F
TS22: medial ganglionic eminence	Strong		2F
TS22: hippocampus	Strong		2F

Structure	Level	Pattern	Image	Note
TS27: cortical layer II	Present		3B
TS27: cortical layer III	Present		3B
TS27: cortical layer IV	Present		3B
TS27: cortical subplate	Present		3B
TS27: cerebral cortex ventricular zone	Weak		3B
TS27: hypothalamus	Weak		3B
TS27: epithalamus	Weak		3B	Expression was higher in the epithalamus and the ventral thalamus than in the hypothalamus.
TS27: prethalamus	Weak		3B	Expression was higher in the epithalamus and the ventral thalamus than in the hypothalamus.
TS27: corpus striatum	Present		3B	Expression was detected in all of the nuclei forming the striatum.
TS27: caudate-putamen	Present		3B
TS27: cerebellum	Present		3B
TS27: globus pallidus	Present		3B
TS27: hippocampus	Present		3B
TS27: pons	Present		3B
TS27: substantia nigra	Present		3B
TS27: superior colliculus	Present		3B
TS27: midbrain	Trace		3B

Structure	Level	Pattern	Image	Note
TS27: cortical layer II	Present		3E
TS27: cortical layer III	Present		3E
TS27: cortical layer IV	Present		3E
TS27: cortical subplate	Present		3E
TS27: cerebral cortex ventricular zone	Weak		3E

Structure	Level	Pattern	Image	Note
TS27: cortical layer II	Present		3F
TS27: cortical layer III	Present		3F
TS27: cortical layer IV	Present		3F
TS27: cortical subplate	Present		3F
TS27: cerebral cortex ventricular zone	Weak		3F
TS27: CA1 pyramidal cell layer	Present		3F
TS27: dentate gyrus granule cell layer	Present		3F
TS27: hippocampus CA2	Present		3F
TS27: hippocampus CA3	Present		3F

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present
TS28: cortical layer III	Present
TS28: cortical layer IV	Present
TS28: cortical subplate	Present
TS28: cerebral cortex ventricular zone	Weak

Structure	Level	Pattern	Image	Note
TS28: neocortex layer	Weak	Non-Uniform	3H	Expression was detected in all layers of the cortex.
TS28: cortical layer IV	Present		3H

Structure	Level	Pattern	Image	Note
TS28: basal forebrain	Absent

Structure	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	Present		3J
TS28: CA2 pyramidal cell layer	Present		3J
TS28: CA3 pyramidal cell layer	Present		3J
TS28: dentate gyrus granule cell layer	Present		3J

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	5B	Expression was detected in the rhombic lip.

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Present		5D	All compartments of the developing cerebellum were significantly labeled.

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Present		5F	All compartments of the developing cerebellum were significantly labeled.

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Strong		5H

Structure	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	Weak		6B	Expression was high in the interneurons.

Structure	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	Weak		6D	Expression was high in the interneurons.

Structure	Level	Pattern	Image	Note
TS28: cerebellum molecular layer	Weak		6F	Expression was high in the interneurons.