Probe:	Prune-M1 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Sections were post-fixed in 4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Strong		1A	Very high expression was detected in telencephalic vesicles.
TS17: diencephalon	Present		1A

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Strong		1B	Very high expression was detected in telencephalic vesicles, the primordium of the subpallium, and the hippocampal area.
TS17: midbrain roof plate	Strong		1B
TS17: diencephalon	Present		1B
TS17: hindbrain	Strong	Regionally restricted	1B	Expression was strong in the rhombic lips of the cranial edge of the thinned roof of the fourth ventricle.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Strong		1C	Very high expression was detected in telencephalic vesicles.
TS17: midbrain roof plate	Strong		1C
TS17: hindbrain	Strong	Regionally restricted	1C	Expression was strong in the rhombic lips of the cranial edge of the thinned roof of the fourth ventricle.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Present		2A	Expression was detected in the neopallial cortex in many densely packed cells.
TS22: epithalamus	Strong		2A
TS22: hypothalamus	Strong		2A
TS22: midbrain lateral wall	Very strong		2A
TS22: cerebellum	Present		2A
TS22: midbrain tegmentum	Present		2A

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present		2C	Expression was detected in all layers of the developing telencephalon.
TS22: corpus striatum	Strong		2C
TS22: epithalamus	Strong		2C
TS22: hypothalamus	Strong		2C
TS22: midbrain lateral wall	Very strong		2C
TS22: cerebellum	Present		2C
TS22: midbrain tegmentum	Present		2C

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present		2E	Expression was detected in all layers of the developing telencephalon.
TS22: cerebral cortex intermediate zone	Present		2E
TS22: cerebral cortex marginal layer	Present		2E
TS22: cerebral cortex ventricular layer	Present		2E
TS22: cortical plate	Present		2E
TS22: cortical subplate	Present		2E
TS22: cerebral cortex	Strong		2E	Strong expression was seen in all of the cell bodies forming the cortical developing layers.
TS22: lateral ganglionic eminence	Strong		2E
TS22: medial ganglionic eminence	Strong		2E
TS22: hippocampus	Strong		2E

Structure	Level	Pattern	Image	Note
TS27: cortical layer II	Present		3A
TS27: cortical layer III	Present		3A
TS27: cortical layer IV	Present		3A
TS27: cortical subplate	Present		3A
TS27: cerebral cortex ventricular zone	Weak		3A
TS27: hypothalamus	Weak		3A
TS27: thalamus	Weak		3A
TS27: corpus striatum	Present		3A	Expression was detected in all of the nuclei forming the striatum.
TS27: caudate-putamen	Present		3A
TS27: cerebellum	Present		3A
TS27: globus pallidus	Present		3A
TS27: hippocampus	Present		3A
TS27: pons	Present		3A
TS27: substantia nigra	Present		3A
TS27: superior colliculus	Present		3A
TS27: midbrain tegmentum	Present	Widespread	3A
TS27: tectum	Present	Widespread	3A

Structure	Level	Pattern	Image	Note
TS27: cortical layer II	Present		3C
TS27: cortical layer III	Present		3C
TS27: cortical layer IV	Present		3C
TS27: cortical subplate	Present		3C
TS27: cerebral cortex ventricular zone	Weak		3C

Structure	Level	Pattern	Image	Note
TS27: cortical layer II	Present		3D
TS27: cortical layer III	Present		3D
TS27: cortical layer IV	Present		3D
TS27: cortical subplate	Present		3D
TS27: cerebral cortex ventricular zone	Weak		3D
TS27: CA1 pyramidal cell layer	Present		3D
TS27: dentate gyrus granule cell layer	Present		3D
TS27: hippocampus CA2	Present		3D
TS27: hippocampus CA3	Present		3D

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present
TS28: cortical layer III	Present
TS28: cortical layer IV	Present
TS28: cortical subplate	Present
TS28: cerebral cortex ventricular zone	Weak

Structure	Level	Pattern	Image	Note
TS28: neocortex layer	Present	Non-Uniform	3G	Expression was observed in the cell bodies with variable intensities throughout the different cortical layers.
TS28: cortical layer I	Strong		3G
TS28: cortical layer II	Strong		3G
TS28: cortical layer IV	Strong		3G

Structure	Level	Pattern	Image	Note
TS28: basal forebrain	Absent

Structure	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	Present		3I
TS28: CA2 pyramidal cell layer	Present		3I
TS28: CA3 pyramidal cell layer	Present		3I
TS28: dentate gyrus granule cell layer	Present		3I

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	5A	Expression was detected in the rhombic lip.

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Present		5C	All compartments of the developing cerebellum were significantly labeled.

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Present		5E	All compartments of the developing cerebellum were significantly labeled.

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Strong		5G
TS27: cerebellum Purkinje cell layer	Present		5G	Expression was detected in the Purkinje cells migrating to the cerbellar surface.
TS27: cerebellum	Present	Widespread	5G

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		6A
TS28: cerebellum internal granule cell layer	Strong		6A
TS28: cerebellum molecular layer	Strong		6A	Expression was high in the interneurons.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		6C
TS28: cerebellum internal granule cell layer	Strong		6C
TS28: cerebellum molecular layer	Strong		6C	Expression was high in the interneurons.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		6E
TS28: cerebellum internal granule cell layer	Strong		6E
TS28: cerebellum molecular layer	Strong		6E	Expression was high in the interneurons.