About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:115166 Shibasaki K, et al., Expression of the basic helix-loop-factor Olig2 in the developing retina: Olig2 as a new marker for retinal progenitors and late-born cells. Gene Expr Patterns. 2007 Jan;7(1-2):57-65
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6099125
Gene symbol: Olig2
Gene name: oligodendrocyte transcription factor 2
Antibody: Olig2 antibody
Detection system: Secondary antibody
Results
Specimen 1B2: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: neural retina Present Regionally restricted 1B2 Expression in neuroblast layer.
Specimen 1B4: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: retina nuclear layer Strong Regionally restricted 1B4 Expression expanded in neuroblast layer until P0.
Specimen 1C1: embryonic day 12.5 (more )
Note: Double labeled: green - Olig2; red - BrdU.
Structure Level Pattern Image Note
TS20: neural retina Trace Single cells 1C1" , 1C1 Expression in retinal progenitor cells, co-localizing with BrdU.
Specimen 1C2: embryonic day 14.5 (more )
Note: Double labeled: green - Olig2; red - BrdU.
Structure Level Pattern Image Note
TS22: neural retina Present Regionally restricted 1C2 , 1C2" Expression in retinal progenitor cells, co-localizing with BrdU. Expression expanded into neuroblast layer from E14.5 to P0.
Specimen 1C3: postnatal day 0 (more )
Note: Double labeled: green - Olig2; red - BrdU.
Structure Level Pattern Image Note
TS27: retina nuclear layer Strong Regionally restricted 1C3 , 1C3" Expression expanded into neuroblast layer from E14.5 to P0. Most expression co-localized with Ki67 at P0.
Specimen 2A: embryonic day 14.5 (more )
Note: Double labeled: green - Olig2; red - Mki67 (assay MGI:6100922).
Structure Level Pattern Image Note
TS22: neural retina Present Regionally restricted 2A Merged , 2A Olig2 Expression co-localized with Ki67.
Specimen 2B: embryonic day 18.5 (more )
Note: Double labeled: green - Olig2; red - Mki67 (assay MGI:6100922).
Structure Level Pattern Image Note
TS26: neural retina Present Regionally restricted 2B Merged , 2B Olig2 Expression co-localized with Ki67.
Specimen 2C: postnatal day 4 (more )
Note: Double labeled: green - Olig2; red - Mki67 (assay MGI:6100922).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present 2C Olig2 , 2C Merged Only a few cells co-expressed Ki67. Some expressing cells in inner nuclear layer not co-localized with Ki67.
Specimen 2D: postnatal day 7 (more )
Note: Double labeled: green - Olig2; red - Mki67 (assay MGI:6100922).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present 2D Merged , 2D Olig2
TS28: retina inner nuclear layer Present 2D Merged , 2D Olig2
TS28: retina outer nuclear layer Absent 2D Merged , 2D Olig2
Specimen 2E: embryonic day 14.5 (more )
Note: Double labeled: green - Olig2; red - Pax6 (assay MGI:6100923).
Structure Level Pattern Image Note
TS22: neural retina Present Regionally restricted 2E Olig2 , 2E Merged Most expressing cells in NBL were low Pax6-positive.
Specimen 2F: embryonic day 18.5 (more )
Note: Double labeled: green - Olig2; red - Pax6 (assay MGI:6100923).
Structure Level Pattern Image Note
TS26: neural retina Present Regionally restricted 2F Olig2 , 2F Merged Most expressing cells in NBL were low Pax6-positive.
Specimen 2G: postnatal day 4 (more )
Note: Double labeled: green - Olig2; red - Pax6 (assay MGI:6100923).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Regionally restricted 2G Olig2 , 2G Merged Authors state expression restricted similar to Pax6 patterns.
TS28: retina inner nuclear layer Present Regionally restricted 2G Olig2 , 2G Merged Authors state expression restricted similar to Pax6 patterns.
Specimen 2H: postnatal day 7 (more )
Note: Double labeled: green - Olig2; red - Pax6 (assay MGI:6100923).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Regionally restricted 2H Olig2 , 2H Merged Expression clearly co-localized with Pax6 in amacrine cells.
TS28: retina inner nuclear layer Present Regionally restricted 2H Olig2 , 2H Merged Expression clearly co-localized with Pax6 in amacrine cells.
Specimen 2I: postnatal adult (more )
Note: Double labeled: green - Olig2; red - Pax6 (assay MGI:6100923).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Regionally restricted 2I Olig2 , 2I Merged Expression clearly co-localized with Pax6 in amacrine cells.
TS28: retina inner nuclear layer Present Regionally restricted 2I Olig2 , 2I Merged Expression clearly co-localized with Pax6 in amacrine cells.
Specimen 3A: embryonic day 14.5 (more )
Note: Double labeled: green - Olig2; red - Isl1 (assay MGI:6100924).
Structure Level Pattern Image Note
TS22: neural retina Present Regionally restricted 3A Olig2 , 3A Merged No colocalization with Isl1.
Specimen 3B: embryonic day 18.5 (more )
Note: Double labeled: green - Olig2; red - Isl1 (assay MGI:6100924).
Structure Level Pattern Image Note
TS26: neural retina Present Regionally restricted 3B Olig2 , 3B Merged No colocalization with Isl1.
Specimen 3C: postnatal day 1 (more )
Note: Double labeled: green - Olig2; red - Isl1 (assay MGI:6100924).
Structure Level Pattern Image Note
TS27: retina ganglion cell layer Present Regionally restricted 3C Merged , 3C Olig2 Authors report colocalization with Isl1.
TS27: retina inner nuclear layer Present Regionally restricted 3C Merged , 3C Olig2 Authors report colocalization with Isl1.
Specimen 3D: postnatal day 4 (more )
Note: Double labeled: green - Olig2; red - Isl1 (assay MGI:6100924).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Regionally restricted 3D Olig2 , 3D Merged Expression in migrating cells that were also Isl1-positive.
TS28: retina inner nuclear layer Present Regionally restricted 3D Olig2 , 3D Merged Expression in migrating cells that were also Isl1-positive.
Specimen 3E: postnatal day 7 (more )
Note: Double labeled: green - Olig2; red - Isl1 (assay MGI:6100924).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Regionally restricted 3E Merged , 3E Olig2 Expression in differentiated retinal ganglion cells, amacrine cells and bipolar cells.
TS28: retina inner nuclear layer Present Regionally restricted 3E Merged , 3E Olig2 Expression in differentiated retinal ganglion cells, amacrine cells and bipolar cells.
Specimen 3F: postnatal adult (more )
Note: Double labeled: green - Olig2; red - Isl1 (assay MGI:6100924).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Regionally restricted 3F Olig2 , 3F Merged Expression in differentiated retinal ganglion cells, amacrine cells and bipolar cells.
TS28: retina inner nuclear layer Present Regionally restricted 3F Olig2 , 3F Merged Expression in differentiated retinal ganglion cells, amacrine cells and bipolar cells.
Specimen 3G: embryonic day 14.5 (more )
Note: Double labeled: green - Olig2; red - Brn3a (Pou4f1; assay MGI:6100925).
Structure Level Pattern Image Note
TS22: neural retina Present Regionally restricted 3G Merged , 3G Olig2 No co-expression witn Brn3a (Pou4f1) in neuroblastic layer.
Specimen 3H: embryonic day 18.5 (more )
Note: Double labeled: green - Olig2; red - Brn3a (Pou4f1; assay MGI:6100925).
Structure Level Pattern Image Note
TS26: neural retina Present Regionally restricted 3H Olig2 , 3H Merged No co-expression witn Brn3a (Pou4f1) in neuroblastic layer.
Specimen 3I: postnatal day 1 (more )
Note: Double labeled: green - Olig2; red - Brn3a (Pou4f1; assay MGI:6100925).
Structure Level Pattern Image Note
TS27: retina nuclear layer Present Regionally restricted 3I Merged , 3I Olig2 No co-expression with Brn3a (Pou4f1) in neuroblastic layer.
TS27: retina ganglion cell layer Present Regionally restricted 3I Merged , 3I Olig2 Co expression with Brn3a (Pou4f1) in ganglion cell layer from P1.
Specimen 3J: postnatal day 4 (more )
Note: Double labeled: green - Olig2; red - Brn3a (Pouf41; assay MGI:6100925).
Structure Level Pattern Image Note
TS28: retina nuclear layer Present Regionally restricted 3J Merged , 3J Olig2 No co-expression with Brn3a (Pou4f1) in neuroblastic layer.
TS28: retina ganglion cell layer Present Regionally restricted 3J Olig2 , 3J Merged Co-expression with Brn3a (Pou4f1) in ganglion cell layer.
Specimen 3K: postnatal day 7 (more )
Note: Double labeled: green - Olig2; red - Brn3a (Pou4f1; assay MGI:6100925).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Regionally restricted 3K Olig2 , 3K Merged Co-expression with Brn3a (Pou4f1) in ganglion cell layer.
Specimen 3L: postnatal adult (more )
Note: Double labeled: green - Olig2; red - Brn3a (Pou4f1; assay MGI:6100925).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Regionally restricted 3L Merged , 3L Olig2 Co-expression with Brn3a (Pou4f1) in ganglion cell layer.
Specimen 4A: embryonic day 18.5 (more )
Note: Double labeled: green - Olig2; red - Ccnd3 (assay MGI:6100930).
Structure Level Pattern Image Note
TS26: neural retina Ambiguous 4A Olig2 , 4A Merged
Specimen 4B: postnatal day 4 (more )
Note: Double labeled: green - Olig2; red - Ccnd3 (assay MGI:6100930).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted 4B Olig2 , 4B Merged Co-expression with Ccnd3 in a few Mueller cells in the inner nuclear layer.
Specimen 4C: postnatal day 7 (more )
Note: Double labeled: green - Olig2; red - Ccnd3 (assay MGI:6100930).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted 4C Merged , 4C Olig2 Co-expression with Ccnd3 in a few Mueller cells in the inner nuclear layer.
Specimen 4D: postnatal adult (more )
Note: Double labeled: green - Olig2; red - Ccnd3 (assay MGI:6100930).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted 4D Olig2 , 4D Merged Expression in Mueller cells in adult inner nuclear layer co-localized with Ccnd3.
Specimen 4E: postnatal day 1 (more )
Note: Double labeled: green - Olig2; red - Chx10 (Vsx2; assay MGI:6100928).
Structure Level Pattern Image Note
TS27: retina inner nuclear layer Present Regionally restricted 4E Merged , 4E Olig2 Expression in clusters of cells in the inner nuclear layer layer, co-localized with Chx10 (Vsx2).
Specimen 4F: postnatal day 4 (more )
Note: Double labeled: green - Olig2; red - Chx10 (Vsx2; assay MGI:6100928).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Weak Regionally restricted 4F Olig2 , 4F Merged Authors state expressing cell clusters gradually cleared during development towards adult.
Specimen 4G: postnatal day 7 (more )
Note: Double labeled: green - Olig2; red - Chx10 (Vsx2; assay MGI:6100928).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Weak Regionally restricted 4G Olig2 , 4G Merged Authors state expressing cell clusters gradually cleared during development towards adult.
Specimen 4H: postnatal adult (more )
Note: Double labeled: green - Olig2; red - Chx10 (Vsx2; assay MGI:6100928).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted 4H Merged , 4H Olig2 Expression in bipolar cells, mostly co-expressed with Chx10 (Vsx2).
Specimen 4I: embryonic day 18.5 (more )
Note: Double labeled: green - Olig2; red - Calb1 (assay MGI:6100926).
Structure Level Pattern Image Note
TS26: neural retina Ambiguous 4I Olig2 , 4I Merged
Specimen 4J: postnatal day 4 (more )
Note: Double labeled: green - Olig2; red - Calb1 (assay MGI:6100926).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Regionally restricted 4J Merged , 4J Olig2 Partial co-localization with Chx10 (Vsx2) in inner and outer INL and GCL.
TS28: retina inner nuclear layer Present Regionally restricted 4J Merged , 4J Olig2 Partial co-localization with Chx10 (Vsx2) in inner and outer INL and GCL.
Specimen 4K: postnatal day 7 (more )
Note: Double labeled: green - Olig2; red - Calb1 (assay MGI:6100926).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted 4K Merged , 4K Olig2 Co-localization with Calb1 in amacrine cells in inner INL and horizontal cells in outer INL.
TS28: retina ganglion cell layer Present Regionally restricted 4K Merged , 4K Olig2 Co-localization with amacrine cells in GCL.
Specimen 4L: postnatal adult (more )
Note: Double labeled: green - Olig2; red - Calb1 (assay MGI:6100926).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted 4L Merged , 4L Olig2 Co-localization with Calb1 in amacrine cells in inner INL and horizontal cells in outer INL
TS28: retina ganglion cell layer Present Regionally restricted 4L Olig2 , 4L Merged Co-localization with amacrine cells in GCL.
Specimen 4M: postnatal adult (more )
Note: Double labeled: green - Olig2; red - GAD65 (Gad2; assay MGI:6100931).
Structure Level Pattern Image Note
TS28: retina Present Regionally restricted 4M Olig2 , 4M Merged Authors report co-localization with GAD65 (Gad2) in most GABAergic amacrine cells.
Specimen 4N: postnatal adult (more )
Note: Double labeled: green - Olig2; red - Chat (assay MGI:6100932).
Structure Level Pattern Image Note
TS28: retina Present Regionally restricted 4N Merged , 4N Olig2 Authors report co-localization with Chat in most starburst amacrine cells.
Specimen S1A: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: retina nuclear layer Present Regionally restricted S1A Clear nuclear expresssion in neuroblastic layer.
Specimen S1B: embryonic day 17.5; Olig2tm1(cre/Esr1*)Htak/Olig2tm1(cre/Esr1*)Htak (more )
Structure Level Pattern Image Note
TS25: retina Absent S1B
Specimen S1C: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: spinal cord Present Regionally restricted S1C Clear nuclear expresssion in spinal cord.
Specimen S1D: embryonic day 17.5; Olig2tm1(cre/Esr1*)Htak/Olig2tm1(cre/Esr1*)Htak (more )
Structure Level Pattern Image Note
TS25: spinal cord Absent S1D
Specimen S2A: postnatal day 2 (more )
Note: Double labeled: green - Olig2; red - BrdU.
Structure Level Pattern Image Note
TS27: retina inner nuclear layer Present S2A Merged , S2A Olig2
TS27: retina ganglion cell layer Present Regionally restricted S2A Olig2 , S2A Merged Expression in a few BrdU-positive cells in the ganglion cell layer.
Specimen S2B: postnatal day 3 (more )
Note: Double labeled: green - Olig2; red - BrdU.
Structure Level Pattern Image Note
TS27: retina inner nuclear layer Present S2B Olig2 , S2B Merged
TS27: retina ganglion cell layer Present Regionally restricted S2B Olig2 , S2B Merged Expression in a few BrdU-positive cells in the ganglion cell layer.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/07/2024
MGI 6.23 		The Jackson Laboratory