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Gene Expression Data
Assay Details
Assay
Reference: J:115166 Shibasaki K, et al., Expression of the basic helix-loop-factor Olig2 in the developing retina: Olig2 as a new marker for retinal progenitors and late-born cells. Gene Expr Patterns. 2007 Jan;7(1-2):57-65
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6099125
Gene symbol: Olig2
Gene name: oligodendrocyte transcription factor 2
Antibody: anti-Olig2
Detection system: Secondary antibody
Results
Specimen 1B2: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: neural retina Present Regionally restricted 1B2 Expression in neuroblast layer.
Specimen 1B4: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: retina nuclear layer Strong Regionally restricted 1B4 Expression expanded in neuroblast layer until P0.
Specimen 1C1: embryonic day 12.5 (more )
Note: Double labeled: green - Olig2; red - BrdU.
Structure Level Pattern Image Note
TS20: neural retina Trace Single cells 1C1" , 1C1 Expression in retinal progenitor cells, co-localizing with BrdU.
Specimen 1C2: embryonic day 14.5 (more )
Note: Double labeled: green - Olig2; red - BrdU.
Structure Level Pattern Image Note
TS22: neural retina Present Regionally restricted 1C2 , 1C2" Expression in retinal progenitor cells, co-localizing with BrdU. Expression expanded into neuroblast layer from E14.5 to P0.
Specimen 1C3: postnatal day 0 (more )
Note: Double labeled: green - Olig2; red - BrdU.
Structure Level Pattern Image Note
TS27: retina nuclear layer Strong Regionally restricted 1C3 , 1C3" Expression expanded into neuroblast layer from E14.5 to P0. Most expression co-localized with Ki67 at P0.
Specimen 2A: embryonic day 14.5 (more )
Note: Double labeled: green - Olig2; red - Mki67 (assay MGI:6100922).
Structure Level Pattern Image Note
TS22: neural retina Present Regionally restricted 2A Merged , 2A Olig2 Expression co-localized with Ki67.
Specimen 2B: embryonic day 18.5 (more )
Note: Double labeled: green - Olig2; red - Mki67 (assay MGI:6100922).
Structure Level Pattern Image Note
TS26: neural retina Present Regionally restricted 2B Merged , 2B Olig2 Expression co-localized with Ki67.
Specimen 2C: postnatal day 4 (more )
Note: Double labeled: green - Olig2; red - Mki67 (assay MGI:6100922).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present 2C Olig2 , 2C Merged Only a few cells co-expressed Ki67. Some expressing cells in inner nuclear layer not co-localized with Ki67.
Specimen 2D: postnatal day 7 (more )
Note: Double labeled: green - Olig2; red - Mki67 (assay MGI:6100922).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present 2D Merged , 2D Olig2
TS28: retina inner nuclear layer Present 2D Merged , 2D Olig2
TS28: retina outer nuclear layer Absent 2D Merged , 2D Olig2
Specimen 2E: embryonic day 14.5 (more )
Note: Double labeled: green - Olig2; red - Pax6 (assay MGI:6100923).
Structure Level Pattern Image Note
TS22: neural retina Present Regionally restricted 2E Olig2 , 2E Merged Most expressing cells in NBL were low Pax6-positive.
Specimen 2F: embryonic day 18.5 (more )
Note: Double labeled: green - Olig2; red - Pax6 (assay MGI:6100923).
Structure Level Pattern Image Note
TS26: neural retina Present Regionally restricted 2F Olig2 , 2F Merged Most expressing cells in NBL were low Pax6-positive.
Specimen 2G: postnatal day 4 (more )
Note: Double labeled: green - Olig2; red - Pax6 (assay MGI:6100923).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Regionally restricted 2G Olig2 , 2G Merged Authors state expression restricted similar to Pax6 patterns.
TS28: retina inner nuclear layer Present Regionally restricted 2G Olig2 , 2G Merged Authors state expression restricted similar to Pax6 patterns.
Specimen 2H: postnatal day 7 (more )
Note: Double labeled: green - Olig2; red - Pax6 (assay MGI:6100923).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Regionally restricted 2H Olig2 , 2H Merged Expression clearly co-localized with Pax6 in amacrine cells.
TS28: retina inner nuclear layer Present Regionally restricted 2H Olig2 , 2H Merged Expression clearly co-localized with Pax6 in amacrine cells.
Specimen 2I: postnatal adult (more )
Note: Double labeled: green - Olig2; red - Pax6 (assay MGI:6100923).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Regionally restricted 2I Olig2 , 2I Merged Expression clearly co-localized with Pax6 in amacrine cells.
TS28: retina inner nuclear layer Present Regionally restricted 2I Olig2 , 2I Merged Expression clearly co-localized with Pax6 in amacrine cells.
Specimen 3A: embryonic day 14.5 (more )
Note: Double labeled: green - Olig2; red - Isl1 (assay MGI:6100924).
Structure Level Pattern Image Note
TS22: neural retina Present Regionally restricted 3A Olig2 , 3A Merged No colocalization with Isl1.
Specimen 3B: embryonic day 18.5 (more )
Note: Double labeled: green - Olig2; red - Isl1 (assay MGI:6100924).
Structure Level Pattern Image Note
TS26: neural retina Present Regionally restricted 3B Olig2 , 3B Merged No colocalization with Isl1.
Specimen 3C: postnatal day 1 (more )
Note: Double labeled: green - Olig2; red - Isl1 (assay MGI:6100924).
Structure Level Pattern Image Note
TS27: retina ganglion cell layer Present Regionally restricted 3C Merged , 3C Olig2 Authors report colocalization with Isl1.
TS27: retina inner nuclear layer Present Regionally restricted 3C Merged , 3C Olig2 Authors report colocalization with Isl1.
Specimen 3D: postnatal day 4 (more )
Note: Double labeled: green - Olig2; red - Isl1 (assay MGI:6100924).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Regionally restricted 3D Olig2 , 3D Merged Expression in migrating cells that were also Isl1-positive.
TS28: retina inner nuclear layer Present Regionally restricted 3D Olig2 , 3D Merged Expression in migrating cells that were also Isl1-positive.
Specimen 3E: postnatal day 7 (more )
Note: Double labeled: green - Olig2; red - Isl1 (assay MGI:6100924).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Regionally restricted 3E Merged , 3E Olig2 Expression in differentiated retinal ganglion cells, amacrine cells and bipolar cells.
TS28: retina inner nuclear layer Present Regionally restricted 3E Merged , 3E Olig2 Expression in differentiated retinal ganglion cells, amacrine cells and bipolar cells.
Specimen 3F: postnatal adult (more )
Note: Double labeled: green - Olig2; red - Isl1 (assay MGI:6100924).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Regionally restricted 3F Olig2 , 3F Merged Expression in differentiated retinal ganglion cells, amacrine cells and bipolar cells.
TS28: retina inner nuclear layer Present Regionally restricted 3F Olig2 , 3F Merged Expression in differentiated retinal ganglion cells, amacrine cells and bipolar cells.
Specimen 3G: embryonic day 14.5 (more )
Note: Double labeled: green - Olig2; red - Brn3a (Pou4f1; assay MGI:6100925).
Structure Level Pattern Image Note
TS22: neural retina Present Regionally restricted 3G Merged , 3G Olig2 No co-expression witn Brn3a (Pou4f1) in neuroblastic layer.
Specimen 3H: embryonic day 18.5 (more )
Note: Double labeled: green - Olig2; red - Brn3a (Pou4f1; assay MGI:6100925).
Structure Level Pattern Image Note
TS26: neural retina Present Regionally restricted 3H Olig2 , 3H Merged No co-expression witn Brn3a (Pou4f1) in neuroblastic layer.
Specimen 3I: postnatal day 1 (more )
Note: Double labeled: green - Olig2; red - Brn3a (Pou4f1; assay MGI:6100925).
Structure Level Pattern Image Note
TS27: retina nuclear layer Present Regionally restricted 3I Merged , 3I Olig2 No co-expression with Brn3a (Pou4f1) in neuroblastic layer.
TS27: retina ganglion cell layer Present Regionally restricted 3I Merged , 3I Olig2 Co expression with Brn3a (Pou4f1) in ganglion cell layer from P1.
Specimen 3J: postnatal day 4 (more )
Note: Double labeled: green - Olig2; red - Brn3a (Pouf41; assay MGI:6100925).
Structure Level Pattern Image Note
TS28: retina nuclear layer Present Regionally restricted 3J Merged , 3J Olig2 No co-expression with Brn3a (Pou4f1) in neuroblastic layer.
TS28: retina ganglion cell layer Present Regionally restricted 3J Olig2 , 3J Merged Co-expression with Brn3a (Pou4f1) in ganglion cell layer.
Specimen 3K: postnatal day 7 (more )
Note: Double labeled: green - Olig2; red - Brn3a (Pou4f1; assay MGI:6100925).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Regionally restricted 3K Olig2 , 3K Merged Co-expression with Brn3a (Pou4f1) in ganglion cell layer.
Specimen 3L: postnatal adult (more )
Note: Double labeled: green - Olig2; red - Brn3a (Pou4f1; assay MGI:6100925).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Regionally restricted 3L Merged , 3L Olig2 Co-expression with Brn3a (Pou4f1) in ganglion cell layer.
Specimen 4A: embryonic day 18.5 (more )
Note: Double labeled: green - Olig2; red - Ccnd3 (assay MGI:6100930).
Structure Level Pattern Image Note
TS26: neural retina Ambiguous 4A Olig2 , 4A Merged
Specimen 4B: postnatal day 4 (more )
Note: Double labeled: green - Olig2; red - Ccnd3 (assay MGI:6100930).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted 4B Olig2 , 4B Merged Co-expression with Ccnd3 in a few Mueller cells in the inner nuclear layer.
Specimen 4C: postnatal day 7 (more )
Note: Double labeled: green - Olig2; red - Ccnd3 (assay MGI:6100930).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted 4C Merged , 4C Olig2 Co-expression with Ccnd3 in a few Mueller cells in the inner nuclear layer.
Specimen 4D: postnatal adult (more )
Note: Double labeled: green - Olig2; red - Ccnd3 (assay MGI:6100930).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted 4D Olig2 , 4D Merged Expression in Mueller cells in adult inner nuclear layer co-localized with Ccnd3.
Specimen 4E: postnatal day 1 (more )
Note: Double labeled: green - Olig2; red - Chx10 (Vsx2; assay MGI:6100928).
Structure Level Pattern Image Note
TS27: retina inner nuclear layer Present Regionally restricted 4E Merged , 4E Olig2 Expression in clusters of cells in the inner nuclear layer layer, co-localized with Chx10 (Vsx2).
Specimen 4F: postnatal day 4 (more )
Note: Double labeled: green - Olig2; red - Chx10 (Vsx2; assay MGI:6100928).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Weak Regionally restricted 4F Olig2 , 4F Merged Authors state expressing cell clusters gradually cleared during development towards adult.
Specimen 4G: postnatal day 7 (more )
Note: Double labeled: green - Olig2; red - Chx10 (Vsx2; assay MGI:6100928).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Weak Regionally restricted 4G Olig2 , 4G Merged Authors state expressing cell clusters gradually cleared during development towards adult.
Specimen 4H: postnatal adult (more )
Note: Double labeled: green - Olig2; red - Chx10 (Vsx2; assay MGI:6100928).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted 4H Merged , 4H Olig2 Expression in bipolar cells, mostly co-expressed with Chx10 (Vsx2).
Specimen 4I: embryonic day 18.5 (more )
Note: Double labeled: green - Olig2; red - Calb1 (assay MGI:6100926).
Structure Level Pattern Image Note
TS26: neural retina Ambiguous 4I Olig2 , 4I Merged
Specimen 4J: postnatal day 4 (more )
Note: Double labeled: green - Olig2; red - Calb1 (assay MGI:6100926).
Structure Level Pattern Image Note
TS28: retina ganglion cell layer Present Regionally restricted 4J Merged , 4J Olig2 Partial co-localization with Chx10 (Vsx2) in inner and outer INL and GCL.
TS28: retina inner nuclear layer Present Regionally restricted 4J Merged , 4J Olig2 Partial co-localization with Chx10 (Vsx2) in inner and outer INL and GCL.
Specimen 4K: postnatal day 7 (more )
Note: Double labeled: green - Olig2; red - Calb1 (assay MGI:6100926).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted 4K Merged , 4K Olig2 Co-localization with Calb1 in amacrine cells in inner INL and horizontal cells in outer INL.
TS28: retina ganglion cell layer Present Regionally restricted 4K Merged , 4K Olig2 Co-localization with amacrine cells in GCL.
Specimen 4L: postnatal adult (more )
Note: Double labeled: green - Olig2; red - Calb1 (assay MGI:6100926).
Structure Level Pattern Image Note
TS28: retina inner nuclear layer Present Regionally restricted 4L Merged , 4L Olig2 Co-localization with Calb1 in amacrine cells in inner INL and horizontal cells in outer INL
TS28: retina ganglion cell layer Present Regionally restricted 4L Olig2 , 4L Merged Co-localization with amacrine cells in GCL.
Specimen 4M: postnatal adult (more )
Note: Double labeled: green - Olig2; red - GAD65 (Gad2; assay MGI:6100931).
Structure Level Pattern Image Note
TS28: retina Present Regionally restricted 4M Olig2 , 4M Merged Authors report co-localization with GAD65 (Gad2) in most GABAergic amacrine cells.
Specimen 4N: postnatal adult (more )
Note: Double labeled: green - Olig2; red - Chat (assay MGI:6100932).
Structure Level Pattern Image Note
TS28: retina Present Regionally restricted 4N Merged , 4N Olig2 Authors report co-localization with Chat in most starburst amacrine cells.
Specimen S1A: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: retina nuclear layer Present Regionally restricted S1A Clear nuclear expresssion in neuroblastic layer.
Specimen S1B: embryonic day 17.5; Olig2tm1(cre/Esr1*)Htak/Olig2tm1(cre/Esr1*)Htak (more )
Structure Level Pattern Image Note
TS25: retina Absent S1B
Specimen S1C: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: spinal cord Present Regionally restricted S1C Clear nuclear expresssion in spinal cord.
Specimen S1D: embryonic day 17.5; Olig2tm1(cre/Esr1*)Htak/Olig2tm1(cre/Esr1*)Htak (more )
Structure Level Pattern Image Note
TS25: spinal cord Absent S1D
Specimen S2A: postnatal day 2 (more )
Note: Double labeled: green - Olig2; red - BrdU.
Structure Level Pattern Image Note
TS27: retina inner nuclear layer Present S2A Merged , S2A Olig2
TS27: retina ganglion cell layer Present Regionally restricted S2A Olig2 , S2A Merged Expression in a few BrdU-positive cells in the ganglion cell layer.
Specimen S2B: postnatal day 3 (more )
Note: Double labeled: green - Olig2; red - BrdU.
Structure Level Pattern Image Note
TS27: retina inner nuclear layer Present S2B Olig2 , S2B Merged
TS27: retina ganglion cell layer Present Regionally restricted S2B Olig2 , S2B Merged Expression in a few BrdU-positive cells in the ganglion cell layer.
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03/25/2025
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