Gene Expression Data
Assay Details
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Assay
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Reference:
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J:231878
Much C, et al., Endogenous Mouse Dicer Is an Exclusively Cytoplasmic Protein. PLoS Genet. 2016 Jun;12(6):e1006095
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6099082
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Gene symbol:
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Dicer1
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Gene name:
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dicer 1, ribonuclease type III
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Reporter:
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HA
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Antibody:
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anti-HA (MMS-101P)
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Assay notes:
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The authors did not specify which antibody was used, MGI:6099081 may have been used as well. Confocal immunofluorescence.
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Results
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Specimen
1F:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Dicer1tm1.1Doca/Dicer1tm1.1Doca
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
1H:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Dicer1tm1.1Doca/Dicer1tm1.1Doca
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3C:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Dicer1tm1.1Doca/Dicer1tm1.1Doca
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3D:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Dicer1tm1.1Doca/Dicer1tm1.1Doca
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5A:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Dicer1tm1.1Doca/Dicer1tm1.1Doca
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: embryo |
Present |
Ubiquitous |
5A
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Expression varies slightly between the tissues.
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Specimen
5B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Dicer1tm1.1Doca/Dicer1tm1.1Doca
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5C:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Dicer1tm1.1Doca/Dicer1tm1.1Doca
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5D:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Dicer1tm1.1Doca/Dicer1tm1.1Doca
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5E:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Dicer1tm1.1Doca/Dicer1tm1.1Doca
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5F:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Dicer1tm1.1Doca/Dicer1tm1.1Doca
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5G:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Dicer1tm1.1Doca/Dicer1tm1.1Doca
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5H:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Dicer1tm1.1Doca/Dicer1tm1.1Doca
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5I:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Dicer1tm1.1Doca/Dicer1tm1.1Doca
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
S1 Testis:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Dicer1tm1.1Doca/Dicer1tm1.1Doca
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
S1 Thymus:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Dicer1tm1.1Doca/Dicer1tm1.1Doca
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Analysis Tools
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