Summary | Mutation origin | Mutation description | Find Mice (IMSR) | Notes | References

Symbol:	Gt(ROSA)26Sortm1(CAG-GFP,-Mars*L274G)Esm
Name:	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Erin M Schuman
MGI ID:	MGI:6095164
Synonyms:	R26-MetRS*, STOPFLOXR26-MetRS*
Gene:	Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance:	Gt(ROSA)26Sortm1(CAG-GFP,-Mars*L274G)Esm page

Germline Transmission:	Earliest citation of germline transmission: J:248509
Parent Cell Line:	Other (see notes) (ES Cell)
Strain of Origin:	C57BL/6NTac

Allele Type:    Targeted (Conditional ready, Inserted expressed sequence, Reporter) Mutation:    Insertion   Gt(ROSA)26Sortm1(CAG-GFP,-Mars*L274G)Esm expresses 1 gene Knock-in expresses: Organism Expressed Gene Note mouse Mars1 (MGI:1345633) mutated mouse gene resulting in L274G substitution   Mutation details: The exchange vector is designed to contain a mutant F3 FRT site, a NEO selection cassette, CAG promoter, a floxed STOP cassette, Green Fluorescent Protein coding sequence, foot-and-mouth disease virus 2A peptide sequence (to mediate ribosomal skipping), the mouse methionine-tRNA synthetase (Mars) with the L274G point mutation, and an FRT site. This vector was integrated into the Gt(ROSA)26Sor locus by recombinase mediated cassette exchange (RMCE). The L274G mutation allows the binding of the methionine surrogate azidonorleucine (ANL) onto the methionine tRNA. ANL can be tagged for imaging or affinity purification. (J:248509)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:	944 strains or lines available

Original:	J:248509 Alvarez-Castelao B, et al., Cell-type-specific metabolic labeling of nascent proteomes in vivo. Nat Biotechnol. 2017 Dec;35(12):1196-1201
All:	4 reference(s)