Antibody:	anti-trpE-Nuk
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS12: future midbrain	Strong		3B
TS12: rhombomere	Strong	Regionally restricted	3B	Expressed in r2/r3.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Strong	Regionally restricted	3C	Expressed in the ventral region.
TS13: future prosencephalon neural fold	Strong	Regionally restricted	3C	Expressed in the ventral region.
TS13: rhombomere 2	Strong		3C
TS13: rhombomere 3	Strong		3C
TS13: rhombomere 5	Strong		3C
TS13: sinus venosus	Present		3C

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Very strong	Regionally restricted	3D left , 3E	Expressed in the ventral region.
TS15: optic cup outer layer	Present		3D left , 3E
TS15: rhombomere 2	Present		3D left , 3E
TS15: telencephalon	Present	Regionally restricted	3E , 3D left	Expressed in basal region.
TS15: eye	Very strong	Regionally restricted	3D left , 3E	Expressed in the optic chiasm.
TS15: future hindbrain floor plate	Present		3D left , 3E	Expressed in the ventral region.
TS15: optic stalk	Present		3D left , 3E	Expressed in the ventral region.
TS15: rhombomere 4 lateral wall	Present	Regionally restricted	3E , 3D left	Expressed in a small patch just anterior of the otic vesicle.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Very strong	Regionally restricted	3F	Expressed in morphological bulge corresponding to the ventral/basal plate. Expressed in all layers.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Very strong	Regionally restricted	3G	Expressed in morphological bulge corresponding to the ventral/basal plate. Expressed in all layers.

Structure	Level	Pattern	Image	Note
TS17: midbrain	Very strong	Regionally restricted	3H	Expressed in the ventral region.
TS17: hindbrain floor plate	Present		3H
TS17: eye	Very strong	Regionally restricted	3H	Expressed in the optic chiasm.
TS17: diencephalon	Absent		3H

Structure	Level	Pattern	Image	Note
TS17: midbrain	Very strong	Regionally restricted	3I	Expressed in morphological bulge corresponding to the ventral/basal plate.
TS17: hindbrain floor plate	Present		3I	Expressed at lower levels.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Regionally restricted	4A	Expressed in the dorsal-most cells where they make contact with the surrounding mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present	Regionally restricted	4C	Expressed at the sites of cell-cell contact at the proliferative ventricular zone.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present	Regionally restricted	4D	Expressed at some of the sites of cell-cell contact at the postmitotic mantle region.

Structure	Level	Pattern	Image	Note
TS18: cranial nerve	Strong	Regionally restricted	5A	Expressed in the oculomotor nerve.
TS18: brain	Present	Regionally restricted	5A	Expressed in a connection between the telencephalon and the midbrain.

Structure	Level	Pattern	Image	Note
TS18: cranial nerve	Present	Regionally restricted	5B	Expressed the oculomotor nerve.
TS18: midbrain	Present		5B
TS18: brain	Present	Regionally restricted	5B	Expressed in an early pathway between the telencephalon and the midbrain.

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Present	Regionally restricted	5C	Expressed in oculomotor nerve.

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Present	Regionally restricted	5E	Expressed in the oculomotor nerve.
TS17: midbrain	Present	Regionally restricted	5E	Expressed in the ventral region.

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Present	Regionally restricted	5F	Expressed in oculomotor axon fascicule.
TS17: retina	Strong		5F

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Strong	Regionally restricted	6A	Expressed in the trigeminal and facial nerve fibers.

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Strong	Regionally restricted	6B	Expressed in facial nerve axon fascicules.
TS17: hindbrain	Present	Regionally restricted	6B	Expressed in the caudal region.

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Strong	Regionally restricted	6C	Expressed in facial nerve axon fascicules.
TS17: hindbrain	Present	Regionally restricted	6C	Expressed in the caudal region.

Structure	Level	Pattern	Image	Note
TS19: accessory nerve	Present		6D
TS19: hypoglossal nerve	Present		6D
TS19: vagus nerve	Present		6D

Structure	Level	Pattern	Image	Note
TS19: accessory nerve	Present		6E
TS19: hypoglossal nerve	Present		6E
TS19: vagus nerve	Present		6E

Structure	Level	Pattern	Image	Note
TS16: spinal ganglion or nerve	Strong	Regionally restricted	6F	Expressed in the earliest spinal motor axons as they exit the neural tube.

Structure	Level	Pattern	Image	Note
TS17: spinal nerve	Present		6G , 6G insert	Expressed throughout leading tips of the growth cones.

Structure	Level	Pattern	Image	Note
TS18: spinal nerve	Present		6H	Expressed in spinal cord ventral motor axons.
TS18: accessory nerve	Present		6H
TS18: neural tube	Present	Uniform	6H

Structure	Level	Pattern	Image	Note
TS18: spinal nerve	Present		6I	Expressed in spinal cord ventral motor axons.
TS18: accessory nerve	Present		6I
TS18: neural tube	Present	Uniform	6I

Structure	Level	Pattern	Image	Note
TS18: spinal nerve	Present	Regionally restricted	6J	Expressed in spinal motor nerve as they elongate to the plexus regions.
TS18: dorsal root ganglion	Present		6J	Expressed in the rostral cell bodies and their fibers connecting to the neural tube.

Structure	Level	Pattern	Image	Note
TS18: spinal nerve	Present	Regionally restricted	6K	Expressed in spinal motor nerve as they elongate to the plexus regions.
TS18: dorsal root ganglion	Present		6K	Expressed in the rostral cell bodies and their fibers connecting to the neural tube.

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present		6L
TS20: parasympathetic ganglion	Present		6L
TS20: spinal nerve	Present		6L

Structure	Level	Pattern	Image	Note
TS17: otocyst	Very strong	Regionally restricted	7A	Expressed in the dorsal region of the otic vesicle surrounding the budding endolymphatic duct.

Structure	Level	Pattern	Image	Note
TS19: otocyst	Very strong	Regionally restricted	7B	Expressed in the area surrounding the endolymphatic duct.
TS19: cranial nerve	Very strong	Regionally restricted	7B	Expressed in cranial nerve VII.

Structure	Level	Pattern	Image	Note
TS19: cranial nerve	Present	Regionally restricted	7C	Expressed in the vestibulocochlear sensory fibers.
TS19: eye	Present		7C

Structure	Level	Pattern	Image	Note
TS19: endolymphatic appendage	Strong	Regionally restricted	7D	Expressed in the membrane of the endolymphatic duct, which makes contact with the surrounding mesenchymal/neural crest cells.
TS19: cranial ganglion	Present	Regionally restricted	7D	Expressed in the vestibulocochlear ganglion.

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Present

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted		Expressed in the ventral region of the thalamus and hypothalamus.

Structure	Level	Pattern	Image	Note
TS16: peripheral nervous system	Present			Highly concentrated within most if not all early axononal projections.
TS16: central nervous system	Present			Expressed in axononal projections of at least one early pathway.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted		Expressed in the ventral region of the thalamus and hypothalamus.
TS17: cranial ganglion	Very strong	Regionally restricted		Expressed in vestibulocochlear ganglion.

Structure	Level	Pattern	Image	Note
TS20: peripheral nervous system	Present			Highly concentrated within most if not all early axononal projections.
TS20: central nervous system	Present			Expressed in axononal projections of at least one early pathway.
TS20: vagus nerve	Present	Regionally restricted		Expressed in the nerve fibers surrounding the heart.
TS20: phrenic nerve	Present	Regionally restricted		Expressed in the nerve fibers surrounding the diaphragm.

Structure	Level	Pattern	Image	Note
TS20: cranial nerve	Absent
TS20: vestibulo-cochlear ganglion	Very strong
TS20: cranial ganglion	Weak	Regionally restricted		Expressed in the acoustic ganglion.
TS20: endolymphatic appendage	Weak