Summary | Mutation origin | Mutation description | Expression | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Chia1tm1.1(icre)Lky
Name:	chitinase, acidic 1; targeted mutation 1.1, Richard M Locksley
MGI ID:	MGI:5927647
Synonyms:	Chia1tm1.1(icre,tdTomato)Lky, ChiaRed
Gene:	Chia1  Location: Chr3:106020698-106039434 bp, + strand  Genetic Position: Chr3, 46.49 cM, cytoband F3
Alliance:	Chia1tm1.1(icre)Lky page

Germline Transmission:	Earliest citation of germline transmission: J:241504
Parent Cell Line:	PRX-B6T (ES Cell)
Strain of Origin:	C57BL/6J

Allele Type:    Targeted (Null/knockout, Recombinase, Reporter) Mutation:    Insertion   Chia1tm1.1(icre)Lky expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Chia1 (MGI:1932052)   Mutation details: A composite fluorescent reporter/recombinase cassette was introduced to exon 1 of the mouse Chia1 gene via homologous recombination embryonic stem (ES) cells. The targeting vector incorporated rabbit beta-globin (exon 2-3 partial genomic sequence serving as an artificial intron and splicing donor/acceptor), tandem tomato (tdTomato) fluorescent reporter, encephalomyocarditic virus (EMCV) internal ribosome entry site (IRES), P1 phage cre recombinase cDNA optimized for mammalian cells (hCre; iCre), bovine growth hormone (bGH) poly-adenylation signal (pA), and a floxed neomycin resistance cassette. The loxP-flanked neomycin cassette was excised from the resultant mice via crosses with B6.C-Tg(CMV-cre)1Cgn/J animal. (J:241504)

Activity:	Tissue activity of this recombinase allele
Driver:	Chia1 (mouse) Summary of all recombinase alleles driven by Chia1. Comparative matrix view of recombinase activities Alliance mouse Chia1 gene page

In Mice Carrying this Mutation:

16 assay results 1 RNA-Seq or microarray experiment(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Chia1 Mutation:	53 strains or lines available

Original:	J:241504 Van Dyken SJ, et al., Spontaneous Chitin Accumulation in Airways and Age-Related Fibrotic Lung Disease. Cell. 2017 Apr 20;169(3):497-509.e13
All:	2 reference(s)