Gene Expression Data
Assay Details
Assay
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Reference:
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J:18498
Schmidt CJ, et al., G(O), a guanine nucleotide binding protein, is expressed during neurite extension in the embryonic mouse. J Neurosci Res. 1994 Jun 1;38(2):182-7
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5925242
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Gene symbol:
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Gap43
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Gene name:
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growth associated protein 43
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Antibody:
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anti-GAP43 (4F11)
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Assay notes:
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Embryos were fixed either with periodate-lysine-paraformaldehyde or in a 9:1 mixture of 4% paraformaldehyde:saturated picric acid.
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Results
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Specimen
E7 not shown:
embryonic day 7.0
(more )
Specimen
E7 not shown:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 7.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
E8 not shown:
embryonic day 8.0
(more )
Specimen
E8 not shown:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
E9 not shown:
embryonic day 9.0
(more )
Specimen
E9 not shown:
(close )
Genetic Background:
|
Not Specified
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Age:
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embryonic day 9.0
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS14: future brain |
Weak |
Regionally restricted |
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Faint expression in marginal zone of neural tube at sites of differentiating neurons.
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Specimen
2B:
embryonic day 10.5
(more )
Specimen
2B:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 10.5
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS17: diencephalon marginal layer |
Strong |
Regionally restricted |
2B
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Intense expression in first post-mitotic neurons in marginal zone of neuronal tube. Expression in both cytoplasm and emerging neurites.
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TS17: Rathke's pouch |
Present |
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2B
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Specimen
E10.5 not shown:
embryonic day 10.5
(more )
Specimen
E10.5 not shown:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 10.5
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
4B:
embryonic day 11.5
(more )
Specimen
4B:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 11.5
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS19: hindbrain marginal layer |
Present |
Uniform |
4B
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Uniform expression in thick layer of post-mitotic neurons in marginal zones of midbrain and hindbrain.
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TS19: midbrain marginal layer |
Present |
Uniform |
4B
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Uniform expression in thick layer of post-mitotic neurons in marginal zones of midbrain and hindbrain.
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Specimen
4D:
embryonic day 11.5
(more )
Specimen
4D:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
E11.5 not shown:
embryonic day 11.5
(more )
Specimen
E11.5 not shown:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 11.5
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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Other - See Notes
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Embedding:
|
Cryosection
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Specimen
5A:
embryonic day 10.5
(more )
Specimen
5A:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 10.5
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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Other - See Notes
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Embedding:
|
Cryosection
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Specimen
5C:
embryonic day 10.5
(more )
Specimen
5C:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 10.5
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
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Embedding:
|
Cryosection
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