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Gene Expression Data
Assay Details
Assay
Reference: J:166346 Tolkunova E, et al., PIAS proteins as repressors of Oct4 function. J Mol Biol. 2007 Dec 14;374(5):1200-12
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5919943
Gene symbol: Pias4
Gene name: protein inhibitor of activated STAT 4
Antibody: anti-PIASy
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 6a: embryonic day 3.5 (more )
Note: Triple labeled: green - Pias4; red - Pou5f1 (assay MGI:5919925); yellow - Cdx2 (assay MGI:5919926).
Structure Level Pattern Image Note
TS4: blastocyst Present Widespread 6a merge , 6a PIASy Expressed in all cells, although a tendency towards its extinction in the inner cells. (Left two embryos in image.)
TS4: compacted morula Present Widespread 6a PIASy Expressed in all cells, although a tendency towards its extinction in the inner cells. (Upper right embryo in image.)
Specimen 6b: embryonic day 3.5 (more )
Note: Triple labeled: green - Pias4; red - Pou5f1 (assay MGI:5919925); yellow - Cdx2 (assay MGI:5919926).
Structure Level Pattern Image Note
TS4: trophectoderm Present 6b merge , 6b PIASy Coexpressed with Pou5f1 in the nuclei.
Specimen 6c: embryonic day 4.5 (more )
Note: Triple labeled: green - Pias4; red - Pou5f1 (assay MGI:5919925); yellow - Cdx2 (assay MGI:5919926).
Structure Level Pattern Image Note
TS6: trophectoderm Present 6c merge , 6c PIASy
Specimen not shown: embryonic day 0.5 (more )
Note: 1 cell stage
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Absent
Specimen not shown: embryonic day 1.0 (more )
Note: 2 cells stage
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Absent
Specimen not shown: embryonic day 2.0 (more )
Note: 4 cells stage
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Absent
Specimen not shown: embryonic day 2.5 (more )
Note: 8-16 cells stage
Structure Level Pattern Image Note
TS3: compacted morula Present Expressed in most cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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