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Gene Expression Data
Assay Details
Assay
Reference: J:166346 Tolkunova E, et al., PIAS proteins as repressors of Oct4 function. J Mol Biol. 2007 Dec 14;374(5):1200-12
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5919925
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: anti-Oct4
Detection system: Secondary antibody coupled to Alexa Fluor 633
Results
Specimen 6a: embryonic day 3.5 (more )
Note: Triple labeled: red - Pou5f1; green - Pias4 (assay MGI:5919943); yellow - Cdx2 (assay MGI:5919926).
Structure Level Pattern Image Note
TS4: blastocyst Present Widespread 6a merge , 6a Oct4 Expressed in all cells. (Left two embryos in image.)
TS4: compacted morula Present Widespread 6a Oct4 , 6a merge Expressed in all cells. (Upper right embryo in image.)
Specimen 6b: embryonic day 3.5 (more )
Note: Triple labeled: red - Pou5f1; green - Pias4 (assay MGI:5919943); yellow - Cdx2 (assay MGI:5919926).
Structure Level Pattern Image Note
TS4: trophectoderm Present 6b merge , 6b Oct4 Coexpressed with Pias4 in the nuclei.
Specimen 6c: embryonic day 4.5 (more )
Note: Triple labeled: red - Pou5f1; green - Pias4 (assay MGI:5919943); yellow - Cdx2 (assay MGI:5919926).
Structure Level Pattern Image Note
TS6: epiblast Present 6c Oct4 , 6c merge
TS6: primitive endoderm Present 6c Oct4 , 6c merge
TS6: trophectoderm Absent 6c Oct4 , 6c merge
Specimen S1: embryonic day 4.5 (more )
Note: Double labeled: red - Pou5f1; green - Pias3 (assay MGI:5919957).
Structure Level Pattern Image Note
TS6: epiblast Present S1_Oct4 , S1_merge
TS6: primitive endoderm Present S1_Oct4 , S1_merge
TS6: trophectoderm Absent S1_merge , S1_Oct4
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
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