About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:166346 Tolkunova E, et al., PIAS proteins as repressors of Oct4 function. J Mol Biol. 2007 Dec 14;374(5):1200-12
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5919925
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: anti-Oct4
Detection system: Secondary antibody coupled to Alexa Fluor 633
Results
Specimen 6a: embryonic day 3.5 (more )
Note: Triple labeled: red - Pou5f1; green - Pias4 (assay MGI:5919943); yellow - Cdx2 (assay MGI:5919926).
Structure Level Pattern Image Note
TS4: blastocyst Present Widespread 6a merge , 6a Oct4 Expressed in all cells. (Left two embryos in image.)
TS4: compacted morula Present Widespread 6a Oct4 , 6a merge Expressed in all cells. (Upper right embryo in image.)
Specimen 6b: embryonic day 3.5 (more )
Note: Triple labeled: red - Pou5f1; green - Pias4 (assay MGI:5919943); yellow - Cdx2 (assay MGI:5919926).
Structure Level Pattern Image Note
TS4: trophectoderm Present 6b merge , 6b Oct4 Coexpressed with Pias4 in the nuclei.
Specimen 6c: embryonic day 4.5 (more )
Note: Triple labeled: red - Pou5f1; green - Pias4 (assay MGI:5919943); yellow - Cdx2 (assay MGI:5919926).
Structure Level Pattern Image Note
TS6: epiblast Present 6c Oct4 , 6c merge
TS6: primitive endoderm Present 6c Oct4 , 6c merge
TS6: trophectoderm Absent 6c Oct4 , 6c merge
Specimen S1: embryonic day 4.5 (more )
Note: Double labeled: red - Pou5f1; green - Pias3 (assay MGI:5919957).
Structure Level Pattern Image Note
TS6: epiblast Present S1_Oct4 , S1_merge
TS6: primitive endoderm Present S1_Oct4 , S1_merge
TS6: trophectoderm Absent S1_merge , S1_Oct4
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory