Probe:	ephrinA5 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	1a ephrinA5 WT	Expressed in dorsal and ventral limb mesenchyme and the adjacent body wall.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	1a ephrinA5 Mut	Expression is still observed in dorsal limb mesenchyme. In the ventral part, expression is strongly reduced in the regions around the axilla.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present	Regionally restricted	1b ephrinA5 WT	A patterned expression in the dorsal and ventral forelimb.
TS19: dorsal root ganglion	Present		1b ephrinA5 WT
TS19: future spinal cord	Present		1b ephrinA5 WT

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present		1b ephrinA5 Mut	Comparable expression values as in wildtype littermates.
TS19: future spinal cord	Present		1b ephrinA5 Mut	Comparable expression values as in wildtype littermates.
TS19: forelimb bud mesenchyme	Weak	Regionally restricted	1b ephrinA5 Mut	Patterned expression was still observed, with diminished expression in the limb.
TS19: spinal nerve plexus	Weak	Regionally restricted	1b ephrinA5 Mut	Diminished expression within the plexus region.