Tg(ACTB-Wld*)2Cole
Transgene Detail
|
|
| Symbol: |
Tg(ACTB-Wld*)2Cole |
| Name: |
transgene insertion 2, Michael Coleman |
| MGI ID: |
MGI:5911071 |
| Synonyms: |
deltaNLSR213A,R215A WldS, deltaNLS WldS |
| Transgene: |
Tg(ACTB-Wld*)2Cole Location: unknown
|
| Alliance: |
Tg(ACTB-Wld*)2Cole page
|
|
|
|
| Transgene Type: |
|
Transgenic (Inserted expressed sequence) |
| Mutations: |
|
Insertion, Nucleotide substitutions
|
| |
|
Tg(ACTB-Wld*)2Cole expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Note |
| mouse |
Wld (MGI:98952) |
expresses Wlds fusion gene with mutations that disable the NLS |
|
| |
|
Mutation details: A construct consisting of the human beta actin promoter followed by a mutant cDNA for Wlds that contains engineered C>G point mutations changing arginine codons 213 and 215 to alanine (p.Arg213Ala, p.Arg215Ala), was injected into the pronuclei of fertilized (C57BL/6 x CBA)F1 eggs that do not express Wlds. The two mutations weaken or disable the nuclear localization signal, thus changing the localization of the translated peptide towards the cytoplasm. A small percentage of peptides is still directed to the nucleus.
(J:144846)
|
|
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
|
| Original: |
J:144846 Beirowski B, et al., Non-nuclear Wld(S) determines its neuroprotective efficacy for axons and synapses in vivo. J Neurosci. 2009 Jan 21;29(3):653-68 |
| All: |
1 reference(s) |
|
Analysis Tools
