Npr2tm1.1Laj
Targeted Allele Detail
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| Symbol: |
Npr2tm1.1Laj |
| Name: |
natriuretic peptide receptor 2; targeted mutation 1.1, Laurinda A Jaffe |
| MGI ID: |
MGI:5911053 |
| Synonyms: |
Npr2-7E |
| Gene: |
Npr2 Location: Chr4:43631935-43651244 bp, + strand Genetic Position: Chr4, 23.05 cM
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| Alliance: |
Npr2tm1.1Laj page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:228790
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| Parent Cell Line: |
Not Specified (ES Cell)
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| Strain of Origin: |
(129 x C57BL/6J)F1
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| Allele Type: |
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Targeted (Not Applicable) |
| Mutations: |
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Insertion, Nucleotide substitutions
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Mutation details: A floxed construct containing a Npr2 cDNA encoding exons 8- 22, and IRES site, eGFP gene, and FRT flanked pgk-neo casstte was inserted in intron 7 and point mutations in the seven serine and threonine sites in exons 8 and 9 were introduced via homologous recombination. The point mutations converted the serine and threonine codons to glutamate codons. Cre-mediated recombination removed the entire construct. The resulting protein behaves as if it is constitutively phosphorylated.
(J:228790)
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| Original: |
J:228790 Shuhaibar LC, et al., Dephosphorylation of juxtamembrane serines and threonines of the NPR2 guanylyl cyclase is required for rapid resumption of oocyte meiosis in response to luteinizing hormone. Dev Biol. 2016 Jan 1;409(1):194-201 |
| All: |
6 reference(s) |
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Analysis Tools
