Gene Expression Data
Assay Details
Assay
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Reference:
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J:111162
Choe A, et al., Expression patterns of Hox10 paralogous genes during lumbar spinal cord development. Gene Expr Patterns. 2006 Oct;6(7):730-7
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5910224
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Gene symbol:
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Hoxc10
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Gene name:
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homeobox C10
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
5A:
(close )
Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Hoxc10tm1Emca/Hoxc10+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS19: future spinal cord |
Present |
Regionally restricted |
5A
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At L2 level, expression in ventrolateral spinal cord, at lateral edge of spinal cord, and in dorsal ventricular zone.
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Specimen
5B:
(close )
Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Hoxc10tm1Emca/Hoxc10+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS19: future spinal cord |
Present |
Regionally restricted |
5B
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At L4 level, expression in ventrolateral spinal cord, at lateral edge of spinal cord, and in dorsal ventricular zone.
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Specimen
5C:
(close )
Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Hoxc10tm1Emca/Hoxc10+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord lumbar region |
Present |
Regionally restricted |
5C
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At L2 level, dorsal band of expression flanks ventricular zone. No exprssion in ventrolateral spinal cord.
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Specimen
5D:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6J
|
Mutant Allele(s):
|
Hoxc10tm1Emca/Hoxc10+
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord lumbar region |
Present |
Regionally restricted |
5D
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At L4 level, dorsal band of expression flanks ventricular zone. Expression is present in ventrolateral spinal cord.
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Specimen
5E:
(close )
Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Hoxc10tm1Emca/Hoxc10+
|
Age:
|
embryonic day 13.5
|
Sex:
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Not Specified
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Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord lumbar region |
Present |
Regionally restricted |
5E
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At L2 level, prominent expression in ventricular zone. No expression ventrolaterally.
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Specimen
5F:
(close )
Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Hoxc10tm1Emca/Hoxc10+
|
Age:
|
embryonic day 13.5
|
Sex:
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Not Specified
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Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord lumbar region |
Present |
Regionally restricted |
5F
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At L4 level, prominent expression in ventricular zone. Expression present ventrolaterally.
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Specimen
5G:
(close )
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
|
Hoxc10tm1Emca/Hoxc10+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
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Note:
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Double labeled: blue - Hoxc10 reporter; brown - Isl1 (assay MGI:5910226) and Isl2 (assay MGI:5910229).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord lumbar region |
Present |
Regionally restricted |
5G
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At L2 level, little overlap between Hoxc10 reporter expression and Islet-1/2 protein expression at more rostral levels
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Specimen
5G':
(close )
Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
|
Mutant Allele(s):
|
Hoxc10tm1Emca/Hoxc10+
|
Age:
|
embryonic day 13.5
|
Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Hoxc10 reporter; brown - Isl1 (assay MGI:5910226) and Isl2 (assay MGI:5910229).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord lumbar region |
Present |
Regionally restricted |
5G'
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At L2 level, little overlap between Hoxc10 reporter expression and Islet-1/2 protein expression at more rostral levels.
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Specimen
5H:
(close )
Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
|
Hoxc10tm1Emca/Hoxc10+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Hoxc10 reporter; brown - Isl1 (assay MGI:5910226) and Isl2 (assay MGI:5910229).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord lumbar region |
Present |
Regionally restricted |
5H
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At L4 level, Hoxc10 reporter expression overlaps with Islet-1/2 protein expression within the lateral motor column.
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Specimen
5H inset:
embryonic day 13.5; Hoxc10tm1Emca/Hoxc10 +
(more )
Note: Double labeled: blue - Hoxc10 reporter; brown - Isl1 (assay MGI:5910226) and Isl2 (assay MGI:5910229).
Specimen
5H inset:
(close )
Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
|
Hoxc10tm1Emca/Hoxc10+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Hoxc10 reporter; brown - Isl1 (assay MGI:5910226) and Isl2 (assay MGI:5910229).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord lumbar region |
Present |
Regionally restricted |
5H inset
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At L4 level, colocalization of Hoxc10 reporter expression and Islet-1/2 protein expression in some cells.
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Specimen
5I:
(close )
Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Hoxc10tm1Emca/Hoxc10+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: spinal cord lumbar region |
Present |
Regionally restricted |
5I
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At L2 level, expression throughout intermediate gray and along the dorsal midline.
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Specimen
5J:
(close )
Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
|
Hoxc10tm1Emca/Hoxc10+
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: spinal cord lumbar region |
Present |
Regionally restricted |
5J
|
At L4 level, expression throughout intermediate gray and along the dorsal midline.
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