Probe:	Hoxa10 probe4
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	1A	At L1 level, expession in superficial dorsal horn and ventromedial patch.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	1B	At L3 level, expession in superficial dorsal horn and ventromedial patch.

Structure	Level	Pattern	Image	Note
TS20: spinal cord thoracic region	Absent		2A	No expression at thoracic spinal cord levels.

Structure	Level	Pattern	Image	Note
TS20: spinal cord lumbar region	Weak	Regionally restricted	2B	At L1 level, lower levels of expression in rostral lumbar spinal cord. Expression overlapping caudal entent of intermediolateral cell column, dorsally lateral to the ventricular zone, and ventrally adjacent to floorplate.

Structure	Level	Pattern	Image	Note
TS20: spinal cord lumbar region	Present	Regionally restricted	2C	At L2 level, Increasing levels of expression more caudally. Expression dorsally lateral to the ventricular zone, and ventrally adjacent to floorplate.

Structure	Level	Pattern	Image	Note
TS20: spinal cord lumbar region	Present	Regionally restricted	2D	At L4 level, Increasing levels of expression more caudally. Expression dorsally lateral to the ventricular zone, and ventrally adjacent to floorplate. Expression excluded from lateral motor column at caudal levels.

Structure	Level	Pattern	Image	Note
TS21: spinal cord lumbar region	Present	Regionally restricted	3A	At L1 level, lower levels of expression. High levels of expression in caudal intermediolateral cell column, ventromedially adjacent to the floorplate, and at lower levels throughout dorsomedial, intermediate and ventral gray.

Structure	Level	Pattern	Image	Note
TS21: spinal cord lumbar region	Present	Regionally restricted	3B	At L2 level, higher levels of expression. Expression dorsally lateral to ventricular zone.

Structure	Level	Pattern	Image	Note
TS21: spinal cord lumbar region	Present	Regionally restricted	3C	At L4 level, higher levels of expression.

Structure	Level	Pattern	Image	Note
TS23: spinal cord lumbar region	Present	Regionally restricted	4A	At L1 level, expression ventrally in lumbar spinal cord.

Structure	Level	Pattern	Image	Note
TS23: spinal cord lumbar region	Present	Regionally restricted	4B	At L3 level, expression ventrally in lumbar spinal cord. Low levels of expression in dorsal horn.