Probe:	Hoxa5 probe C
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS12: neural tube	Absent		2B
TS12: somite	Absent		2B

Structure	Level	Pattern	Image	Note
TS13: trunk paraxial mesenchyme	Present	Punctate	2D	Expression initiated in posterior region of paraxial mesoderm.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present
TS15: somite	Present

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	2F	Anterior boundary of expression in both central nervous system and somites was more caudal compared to that of the 1.8 kb transcript detected by probe B (see assay MGI:5909948). The anterior boundary of expression in somites was at somite 13-14. The limit of expression in somitic mesoderm was off set caudally compared to that of the neural tube, with an interval of about 10 somites.
TS17: trunk somite	Present	Regionally restricted	2F	Anterior boundary of expression in both central nervous system and somites was more caudal compared to that of the 1.8 kb transcript detected by probe B (see assay MGI:5909948). The anterior boundary of expression in somites was at somite 13-14. The limit of expression in somitic mesoderm was off set caudally compared to that of the neural tube, with an interval of about 10 somites.
TS17: body wall mesenchyme	Absent		2F	No expression in prospective pectoral girdle.
TS17: forelimb bud	Present	Widespread	2F

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	2H	Rostral limit of expression in the central nervous system more caudal compared to that of the 1.8 kb transcript detected by probe C (see assay MGI:5909947).
TS17: trunk somite	Present	Regionally restricted	2H	Rostral limit of expression in the central nervous system more caudal compared to that of the 1.8 kb transcript detected by probe C (see assay MGI:5909947).

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present		2J
TS20: somite	Present		2J
TS20: vertebral pre-cartilage condensation	Present	Regionally restricted	2J	Rostral limit of expression corresponds to prevertebra 10.
TS20: rib pre-cartilage condensation	Weak		2J	Expression in ribs, with lower intensity compared to probe B (see assay MGI:5909948).
TS20: forelimb	Present	Widespread	2J
TS20: hindlimb	Present	Widespread	2J
TS20: stylopod	Weak	Regionally restricted	2J	Expression more faint in zeugopod and stylopod. Expression adjacent to presumptive bones.
TS20: zeugopod	Weak	Regionally restricted	2J	Expression more faint in zeugopod and stylopod. Expression adjacent to presumptive bones.
TS20: limb interdigital region mesenchyme	Present		2J

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present	Regionally restricted	2L	In the central nervous system, rostral limit closer to junction of melencephalon with spinal cord, compared to that seen with probe B (see assay MGI:5909948).
TS20: somite	Present		2L
TS20: vertebral pre-cartilage condensation	Present	Regionally restricted	2L	Rostral limit of expression corresponds to prevertebra 10.