Probe:	Hoxa5 probe B
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present
TS15: somite	Present

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	2E	The limit of expression in somitic mesoderm was off set caudally compared to that of the neural tube, with interval of about 4 somites.
TS17: trunk somite	Present	Regionally restricted	2E	The limit of expression in somitic mesoderm was off set caudally compared to that of the neural tube, with interval of about 4 somites.
TS17: body wall mesenchyme	Present	Regionally restricted	2E	Expression in prospective pectoral girdle.
TS17: forelimb bud	Present	Widespread	2E

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present		2I
TS20: somite	Present		2I
TS20: vertebral pre-cartilage condensation	Present	Regionally restricted	2I	Anterior limit of expression in prevertebral column extends between prevertebra 3 and prevertebra 10.
TS20: rib pre-cartilage condensation	Present		2I
TS20: body wall mesenchyme	Present	Regionally restricted	2I	Expression in prospective pectoral girdle.
TS20: forelimb	Present	Widespread	2I
TS20: hindlimb	Present	Widespread	2I
TS20: stylopod	Weak		2I	Expression more faint in zeugopod and stylopod. Expression adjacent to presumptive bones.
TS20: zeugopod	Weak		2I	Expression more faint in zeugopod and stylopod. Expression adjacent to presumptive bones.
TS20: limb interdigital region mesenchyme	Present		2I

Structure	Level	Pattern	Image	Note
TS20: forelimb interdigital region mesenchyme	Present		6A fl

Structure	Level	Pattern	Image	Note
TS20: hindlimb interdigital region mesenchyme	Present		6A hl