About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:159942 Pike ST, et al., Mitochondrial C1-tetrahydrofolate synthase (MTHFD1L) supports the flow of mitochondrial one-carbon units into the methyl cycle in embryos. J Biol Chem. 2010 Feb 12;285(7):4612-20
Assay type: Northern blot
MGI Accession ID: MGI:5909232
Gene symbol: Mthfd1
Gene name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase
Probe: MTHFD1 (clone 14EB)
Probe preparation: Double stranded labelled with P32 DNA
Assay notes: The results normalized to the ribosomal RNA are illustrated graphically in Fig. 3B.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure 3.6 kb Amount Genetic BackgroundMutant Allele(s)Sex
4.5 E4.5 TS6: embryo Present 20 µg; total RNA Not Specified Not Specified
5.5 E5.5 TS8: embryo Present 20 µg; total RNA Not Specified Not Specified
6.5 E6.5 TS9: embryo Strong 20 µg; total RNA Not Specified Not Specified
7.5 E7.5 TS11: embryo Present 20 µg; total RNA Not Specified Not Specified
8.5 E8.5 TS13: embryo Present 20 µg; total RNA Not Specified Not Specified
9.5 E9.5 TS15: embryo Present 20 µg; total RNA Not Specified Not Specified
10.5 E10.5 TS17: embryo Present 20 µg; total RNA Not Specified Not Specified
11.5 E11.5 TS19: embryo Present 20 µg; total RNA Not Specified Not Specified
12.5 E12.5 TS20: embryo Present 20 µg; total RNA Not Specified Not Specified
13.5 E13.5 TS21: embryo Present 20 µg; total RNA Not Specified Not Specified
14.5 E14.5 TS22: embryo Present 20 µg; total RNA Not Specified Not Specified
15.5 E15.5 TS23: embryo Present 20 µg; total RNA Not Specified Not Specified
16.5 E16.5 TS24: embryo Present 20 µg; total RNA Not Specified Not Specified
17.5 E17.5 TS25: embryo Present 20 µg; total RNA Not Specified Not Specified
18.5 E18.5 TS26: embryo Strong 20 µg; total RNA Not Specified Not Specified
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/16/2024
MGI 6.23 		The Jackson Laboratory