Gene Expression Data
Assay Details
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Assay
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Reference:
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J:44410
Choudhry R, et al., Changing patterns of gap junctional intercellular communication and connexin distribution in mouse epidermis and hair follicles during embryonic development. Dev Dyn. 1997 Dec;210(4):417-30
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5908137
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Gene symbol:
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Gja1
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Gene name:
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gap junction protein, alpha 1
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Antibody:
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anti-connexin 43
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
6A:
embryonic day 12.5
(more  )
Specimen
6A:
(close  )
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Genetic Background:
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ICR
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Acetone
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Embedding:
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Cryosection
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Specimen
6B:
embryonic day 14.0
(more )
Note: stage 2 hair peg
Specimen
6B:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 14.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Acetone
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Embedding:
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Cryosection
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Note:
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stage 2 hair peg
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: epidermis |
Very strong |
Regionally restricted |
6B
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Expression is strongest in the suprabasal layer and at the apical surface of the basal layer.
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Specimen
6C:
embryonic day 14.5
(more )
Note: stage 4 hair peg
Specimen
6C:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Acetone
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Embedding:
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Cryosection
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Note:
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stage 4 hair peg
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: epidermis |
Strong |
Regionally restricted |
6C
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Expression is in the middle layers and is absent in the outermost differentiated layer.
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| TS22: hair follicle |
Present |
Regionally restricted |
6C
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Expression is in the inner core.
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Specimen
6D:
embryonic day 16.5
(more )
Specimen
6D:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Acetone
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Embedding:
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Cryosection
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Specimen
6E:
embryonic day 16.5
(more )
Note: stage 6 follicle
Specimen
6E:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Acetone
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Embedding:
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Cryosection
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Note:
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stage 6 follicle
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Specimen
6F:
embryonic day 16.5
(more )
Note: stage 6 follicle
Specimen
6F:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Acetone
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Embedding:
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Cryosection
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Note:
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stage 6 follicle
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: hair root sheath |
Weak |
Regionally restricted |
6F
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Expression is in keratinocytes. Expression is in Henley's layer and is greatly reduced by the level of mid-bulb.
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Specimen
not shown:
embryonic day 17.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Acetone
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Embedding:
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Cryosection
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Specimen
Table 2 E12-E13:
embryonic day 12.5-13.5
(more )
Note: stage 2-3a hair peg
Specimen
Table 2 E12-E13:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 12.5-13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Acetone
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Embedding:
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Cryosection
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Note:
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stage 2-3a hair peg
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: epidermis |
Present |
Regionally restricted |
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Expression is suprabasal and basal layers in the epidermis, and weaker in the suprabasal layer in hair pegs.
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| TS21: hair follicle |
Absent |
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Specimen
Table 2 E14-E15:
embryonic day 14.5-15.5
(more )
Note: stage 3c-6 hair peg
Specimen
Table 2 E14-E15:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 14.5-15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Acetone
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Embedding:
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Cryosection
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Note:
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stage 3c-6 hair peg
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: epidermis |
Present |
Regionally restricted |
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Expression is suprabasal and basal layers in the epidermis, and weaker in the suprabasal layer in hair pegs.
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| TS23: hair follicle |
Present |
Regionally restricted |
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Expression is in the outer root sheath, in Henley's layer, the germinative matrix, is weaker in the inner root sheath, and Huxley's layer, and is absent in the dermal papilla.
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| TS23: hair |
Weak |
Regionally restricted |
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Expression is in the hair shaft.
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Specimen
Table 2 E16-E17:
embryonic day 16.5-17.5
(more )
Note: stage 3c-6 hair peg
Specimen
Table 2 E16-E17:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 16.5-17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Acetone
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Embedding:
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Cryosection
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Note:
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stage 3c-6 hair peg
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: epidermis |
Present |
Regionally restricted |
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Expression is in the basal layer and is absent in the suprabasal layer.
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| TS25: hair follicle |
Present |
Regionally restricted |
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Expression is in the outer root sheath, in Henley's layer, the germinative matrix, is weaker in the inner root sheath, and Huxley's layer, and is absent in the dermal papilla.
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| TS25: hair |
Weak |
Regionally restricted |
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Expression is in the hair shaft.
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