Probe:	Flk-1 cDNA3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: meninges	Present	Regionally restricted	1A	Expressed in the leptomeninges.
TS20: brain blood vessel	Present		1A
TS20: developing vasculature of metanephros	Present		1A
TS20: dorsal aorta	Present		1A
TS20: intersegmental artery	Present		1A
TS20: intersegmental vein	Present		1A
TS20: lung vascular element	Present		1A
TS20: spinal cord vascular element	Present		1A

Structure	Level	Pattern	Image	Note
TS10: ectoplacental cone	Present		2A
TS10: yolk sac mesoderm	Present		2A
TS10: embryo mesoderm	Present	Regionally restricted	2A	Expressed in the region that is fated to give rise to the heart.

Structure	Level	Pattern	Image	Note
TS12: allantois	Present		3B
TS12: blood island	Present	Regionally restricted	3B	Expressed in the mesodermal layer.
TS12: embryo mesoderm	Present	Regionally restricted	3B	Expressed in the embryonic mesoderm underlying the headfold.

Structure	Level	Pattern	Image	Note
TS12: allantois	Present		3F
TS12: blood island	Present	Regionally restricted	3F	Expressed in the mesodermal layer.
TS12: embryo mesoderm	Present	Regionally restricted	3F	Expressed in the embryonic mesoderm underlying the headfold.

Structure	Level	Pattern	Image	Note
TS13: vascular system	Present	Widespread	4A/B
TS13: dorsal aorta	Present		4A/B
TS13: endocardial tube	Present		4A/B
TS13: amnion	Weak	Regionally restricted	4A/B	Expressed in the mesodermal layer.

Structure	Level	Pattern	Image	Note
TS20: labyrinthine zone	Present	Regionally restricted	5A	Expressed in the endothelial cells.

Structure	Level	Pattern	Image	Note
TS28: placenta	Present		5A

Structure	Level	Pattern	Image	Note
TS20: yolk sac	Present		6A	Expressed at considerably lower levels compared to E8.5.

Structure	Level	Pattern	Image	Note
TS13: yolk sac	Present		6B

Structure	Level	Pattern	Image	Note
TS20: amnion	Absent		6C

Structure	Level	Pattern	Image	Note
TS23: intestine epithelium	Present	Regionally restricted	7B	Expressed in endothelial cells in each villi.
TS23: femur	Present	Regionally restricted	7B	Expressed in endothelial cells.

Structure	Level	Pattern	Image	Note
TS23: renal corpuscle	Strong	Regionally restricted	8B	Expressed in the inner endothelial layer.

Structure	Level	Pattern	Image	Note
TS8: conceptus	Absent

Structure	Level	Pattern	Image	Note
TS11: extraembryonic mesoderm	Present

Structure	Level	Pattern	Image	Note
TS13: trophoblast giant cell	Present

Structure	Level	Pattern	Image	Note
TS22: brain blood vessel	Present
TS22: dorsal aorta	Present
TS22: endocardial lining	Present
TS22: intersegmental artery	Present
TS22: intersegmental vein	Present
TS22: leptomeninges	Present
TS22: lung vascular element	Present
TS22: renal small blood vessel	Present
TS22: spinal cord vascular element	Present
TS22: intestine epithelium	Present	Regionally restricted		Expressed in endothelial cells in each villi.
TS22: renal corpuscle	Present	Regionally restricted		Expressed in the inner endothelial layer.
TS22: femur cartilage condensation	Present	Regionally restricted		Expressed in endothelial cells.

Structure	Level	Pattern	Image	Note
TS23: brain blood vessel	Present
TS23: descending aorta	Present
TS23: endocardial lining	Present
TS23: intersegmental artery	Present
TS23: intersegmental vein	Present
TS23: leptomeninges	Present
TS23: lung vascular element	Present
TS23: renal small blood vessel	Present
TS23: spinal cord vascular element	Present
TS23: choroid plexus	Present	Regionally restricted		Expressed in the inner endothelial layer.