Dixdc1 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:97956 Shiomi K, et al., Identification and differential expression of multiple isoforms of mouse Coiled-coil-DIX1 (Ccd1), a positive regulator of Wnt signaling. Brain Res Mol Brain Res. 2005 Apr 27;135(1-2):169-80
Assay type:	Western blot
MGI Accession ID:	MGI:5907200
Gene symbol:	Dixdc1
Gene name:	DIX domain containing 1

Antibody:	Anti-Ccd1 (C1)
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	A band of about 33 kDa appears to be a degraded fragment or cross-reactivity with an uncharacterized protein. This band was not detected with Andti-Ccd1 (N2) antibody MGI:5902732.

Results

Image: 4A right blot

	Sample Information		Bands			Other Sample Information
Lane	Age	Structure	100 kDa (a)	70 kDa (a)	52 kDa (b)	Genetic Background	Mutant Allele(s)	Sex
B	P w 6	TS28: brain	Absent	Absent	Strong	C57BL/6		Male
L	P w 6	TS28: liver	Absent	Absent	Absent (c)	C57BL/6		Male
S	P w 6	TS28: spleen	Absent	Absent	Ambiguous	C57BL/6		Male
T	P w 6	TS28: testis	Absent	Absent	Strong	C57BL/6		Male
EB	E15.5	TS23: brain	Weak	Weak	Strong	C57BL/6J		Not Specified
EL	E15.5	TS23: liver	Weak	Weak	Ambiguous	C57BL/6J		Not Specified
ES	E15.5	TS23: spleen primordium	Weak	Weak	Ambiguous	C57BL/6J		Not Specified

Notes:
(a) Weak band. Possibly generated from Ccd1A.
(b) Major band. Ccd1B protein and/or the smaller band observed with the recombinant Ccd1A expression.
(c) Absent assigned by curator.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 05/21/2024 MGI 6.23